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Sci Rep

Title:		A novel lung alveolar cell model for exploring volatile biomarkers of particle-induced lung injury
Author(s):		Chuang HC; Tsai SW; Shie RH; Lu YC; Song SR; Huang SH; Peng HY; Yang HY;
Address:		"School of Respiratory Therapy, Taipei Medical University, Taipei, Taiwan. Institute of Environmental and Occupational Health Sciences, National Taiwan University College of Public Health, Taipei, Taiwan. Green Energy and Environmental Research Laboratories, Industrial Technology Research Institute, Hsinchu, Taiwan. Department of Geosciences, National Taiwan University, Taipei, Taiwan. Institute of Environmental and Occupational Health Sciences, National Taiwan University College of Public Health, Taipei, Taiwan. hyang@ntu.edu.tw. Department of Environmental and Occupational Medicine, National Taiwan University Hospital, Taipei, Taiwan. hyang@ntu.edu.tw"
Journal Title:		Sci Rep
Year:		2020
Volume:		20200924
Issue:		1
Page Number:		15700 -
DOI:		10.1038/s41598-020-72825-7
ISSN/ISBN:		2045-2322 (Electronic) 2045-2322 (Linking)
Abstract:		"Quartz can increase oxidative stress, lipid peroxidation, and inflammation. The objective of this study was to explore the volatile biomarkers of quartz-induced lung injury using a lung alveolar cell model. We exposed the human alveolar A549 cell line to 0, 200, and 500 mug/mL quartz particles for 24 h and used gas chromatography-mass spectrometry to measure the volatile metabolites in the headspace air of cells. We identified ten volatile metabolites that had concentration-response relationships with particles exposure, including 1,2,4-oxadiazole, 5-(4-nitrophenyl)-3-phenyl- (CAS: 28825-12-9), 2,6-dimethyl-6-trifluoroacetoxyoctane (CAS: 61986-67-2), 3-buten-1-amine, N,N-dimethyl- (CAS: 55831-89-5), 2-propanol, 2-methyl- (CAS: 75-65-0), glycolaldehyde dimethyl acetal (CAS: 30934-97-5), propanoic acid, 2-oxo-, ethyl ester (CAS: 617-35-6), octane (CAS: 111-65-9), octane, 3,3-dimethyl- (CAS: 4110-44-5), heptane, 2,3-dimethyl- (CAS: 3074-71-3) and ethanedioic acid, bis(trimethylsilyl) ester (CAS: 18294-04-7). The volatile biomarkers are generated through the pathways of propanoate and nitrogen metabolism. The volatile biomarkers of the alkanes and methylated alkanes are related to oxidative and lipid peroxidation of the cell membrane. The lung alveolar cell model has the potential to explore the volatile biomarkers of particulate-induced lung injury"
Keywords:		A549 Cells Alveolar Epithelial Cells/*drug effects/metabolism Biomarkers/metabolism Gas Chromatography-Mass Spectrometry Humans Lung Injury/*metabolism Particulate Matter/*toxicity Quartz/*toxicity Volatile Organic Compounds/*metabolism;
Notes:		"MedlineChuang, Hsiao-Chi Tsai, Shih-Wei Shie, Ruei-Hao Lu, Yi-Chia Song, Sheng-Rong Huang, Sheng-Hsiu Peng, Hsin-Yi Yang, Hsiao-Yu eng Research Support, Non-U.S. Gov't England 2020/09/26 Sci Rep. 2020 Sep 24; 10(1):15700. doi: 10.1038/s41598-020-72825-7"