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Bioengineered

Title:		Recent advances in the production of recombinant subunit vaccines in Pichia pastoris
Author(s):		Wang M; Jiang S; Wang Y;
Address:		"a Institute for Translational Medicine, Medical College of Qingdao University , Qingdao , China. b State Key Laboratory of Virology , College of Life Sciences, Wuhan University , Wuhan , China"
Journal Title:		Bioengineered
Year:		2016
Volume:		7
Issue:		3
Page Number:		155 - 165
DOI:		10.1080/21655979.2016.1191707
ISSN/ISBN:		2165-5987 (Electronic) 2165-5979 (Print) 2165-5979 (Linking)
Abstract:		"Recombinant protein subunit vaccines are formulated using defined protein antigens that can be produced in heterologous expression systems. The methylotrophic yeast Pichia pastoris has become an important host system for the production of recombinant subunit vaccines. Although many basic elements of P. pastoris expression system are now well developed, there is still room for further optimization of protein production. Codon bias, gene dosage, endoplasmic reticulum protein folding and culture condition are important considerations for improved production of recombinant vaccine antigens. Here we comment on current advances in the application of P. pastoris for the synthesis of recombinant subunit vaccines"
Keywords:		"Antigens, Viral/genetics/*immunology Cloning, Molecular Codon/chemistry/immunology Culture Media/chemistry Gene Dosage Gene Expression Genetic Vectors Humans Mating Factor/genetics/metabolism Pichia/*genetics/metabolism Protein Engineering/*methods Protei;"
Notes:		"MedlineWang, Man Jiang, Shuai Wang, Yefu eng Review 2016/06/02 Bioengineered. 2016 Apr; 7(3):155-65. doi: 10.1080/21655979.2016.1191707"