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Yeast

Title:		Postzygotic reproductive isolation among three Saccharomyces yeast species
Author(s):		Toyomura K; Hisatomi T;
Address:		"Department of Biotechnology, Faculty of Life Sciences and Biotechnology, Fukuyama University, Gakuen-cho, Fukuyama, Hiroshima, 729-0292, Japan"
Journal Title:		Yeast
Year:		2021
Volume:		20210129
Issue:		5
Page Number:		326 - 335
DOI:		10.1002/yea.3549
ISSN/ISBN:		1097-0061 (Electronic) 0749-503X (Linking)
Abstract:		"We have previously isolated heterothallic haploid strains from original homothallic diploids of two yeast species in the family Saccharomycetaceae. In this study, heterothallic haploid strains were isolated from an original homothallic diploid of Saccharomyces kudriavzevii type strain, followed by investigation of sexual interactions among these yeast strains, in addition to S. cerevisiae laboratory strains. It has been shown that prezygotic reproductive isolation was observed between Kazachstania naganishii and S. cerevisiae with alpha-factor mating pheromones representing crossaction with each other beyond the genus boundary. Using heterothallic strains, postzygotic reproductive isolation system was shown to reside in the genus Saccharomyces by mass mating and cell-cell contact experiments. In mass mating experiments, crossaction of alpha-factor and a-factor mating pheromones and sexual agglutination effectively occurred beyond species boundaries among S. kudriavzevii, S. paradoxus, and S. cerevisiae. When the fates of cell-cell pairs from these Saccharomyces yeast species were systematically chased one by one, interspecific F1 hybrids were effectively produced, while sporulations were partially prohibited, with spore germination perfectly blocked in the hybrids. These results indicated that postzygotic reproductive isolation definitively resides among these Saccharomyces yeast species and that disorder of chromosome organization had to some extent occurred in interspecific F1 hybrids"
Keywords:		"DNA, Fungal/genetics Haploidy Hybridization, Genetic Phylogeny *Reproductive Isolation Saccharomyces/classification/*genetics/metabolism Saccharomyces cerevisiae/genetics Saccharomycetales/genetics Spores, Fungal Saccharomyces cerevisiae Saccharomyces kud;"
Notes:		"MedlineToyomura, Kousuke Hisatomi, Taisuke eng Research Support, Non-U.S. Gov't England 2021/01/15 Yeast. 2021 May; 38(5):326-335. doi: 10.1002/yea.3549. Epub 2021 Jan 29"