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Proc Natl Acad Sci U S A

Title:		Spatial dynamics of receptor-mediated endocytic trafficking in budding yeast revealed by using fluorescent alpha-factor derivatives
Author(s):		Toshima JY; Toshima J; Kaksonen M; Martin AC; King DS; Drubin DG;
Address:		"Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720-3202, USA"
Journal Title:		Proc Natl Acad Sci U S A
Year:		2006
Volume:		20060330
Issue:		15
Page Number:		5793 - 5798
DOI:		10.1073/pnas.0601042103
ISSN/ISBN:		0027-8424 (Print) 1091-6490 (Electronic) 0027-8424 (Linking)
Abstract:		"Much progress defining the order and timing of endocytic internalization events has come as a result of real-time, live-cell fluorescence microscopy. Although the availability of numerous endocytic mutants makes yeast an especially valuable organism for functional analysis of endocytic dynamics, a serious limitation has been the lack of a fluorescent cargo for receptor-mediated endocytosis. We have now synthesized biologically active fluorescent mating-pheromone derivatives and demonstrated that receptor-mediated endocytosis in budding yeast occurs via the clathrin- and actin-mediated endocytosis pathway. We found that endocytic proteins first assemble into patches on the plasma membrane, and then alpha-factor associates with the patches. Internalization occurs next, concomitant with actin assembly at patches. Additionally, endocytic vesicles move toward early endosomes on actin cables. Early endosomes also associate with actin cables, and they actively move toward endocytic sites to capture vesicles being released from the plasma membrane. Thus, endocytic vesicle formation and capture of the newly released vesicles by early endosomes occur in a highly concerted manner, mediated by the actin cytoskeleton"
Keywords:		Actins/physiology Endocytosis Endosomes/physiology Fluorescent Dyes Mating Factor Peptides/chemistry/*physiology Schizosaccharomyces/*physiology;
Notes:		"MedlineToshima, Junko Y Toshima, Jiro Kaksonen, Marko Martin, Adam C King, David S Drubin, David G eng R01 GM050399/GM/NIGMS NIH HHS/ GM50399/GM/NIGMS NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't 2006/04/01 Proc Natl Acad Sci U S A. 2006 Apr 11; 103(15):5793-8. doi: 10.1073/pnas.0601042103. Epub 2006 Mar 30"