| Title: | Widespread disruption of genomic imprinting in adult interspecies mouse (Mus) hybrids |
| Author(s): | Shi W; Krella A; Orth A; Yu Y; Fundele R; |
| Address: | "Department of Development and Genetics, Evolutionary Biology Center, Uppsala University, Sweden" |
| ISSN/ISBN: | 1526-954X (Print) 1526-954X (Linking) |
| Abstract: | "Mammalian interspecies hybrids exhibit parent-of-origin effects in that offspring of reciprocal matings, even though genetically identical, frequently exhibit opposite phenotypes, especially in growth. This was also observed in hybridization with the genus Mus. These parent-of-origin effects suggested that imbalance in the expression of imprinted genes, which are expressed differentially, depending on their transmission through the maternal or paternal germline, and/or differential loss-of-imprinting (LOI) could underlie these opposite growth phenotypes in reciprocal mammalian hybrids. Here we report that tissue-specific LOI occurs in adult Mus hybrids. Contrary to expectations, LOI patterns were not consistent with a direct influence of altered expression levels of imprinted genes on growth. Bisulfite sequencing revealed that reactivation of maternal alleles of Peg3 and Snrpn in specific tissues was accompanied by partial demethylation at their potential imprinting control regions. We propose that abnormal reprogramming after fertilization and during preimplantation development is in part responsible for hybrid dysgenesis, for which a strong epigenetic basis has been demonstrated" |
| Keywords: | "Animals Chimera/*genetics/growth & development DNA Methylation Epigenesis, Genetic *Genomic Imprinting Growth/genetics Hybridization, Genetic Mice/*genetics/growth & development Mice, Congenic/genetics/growth & development Mice, Inbred C3H Mice, Inbred C5;" |
| Notes: | "MedlineShi, Wei Krella, Antje Orth, Annie Yu, Yang Fundele, Reinald eng Research Support, Non-U.S. Gov't 2005/09/08 Genesis. 2005 Nov; 43(3):100-8. doi: 10.1002/gene.20161" |
