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Proc Natl Acad Sci U S A

Title:		Structure of peptide sex pheromone receptor PrgX and PrgX/pheromone complexes and regulation of conjugation in Enterococcus faecalis
Author(s):		Shi K; Brown CK; Gu ZY; Kozlowicz BK; Dunny GM; Ohlendorf DH; Earhart CA;
Address:		"Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA"
Journal Title:		Proc Natl Acad Sci U S A
Year:		2005
Volume:		20051208
Issue:		51
Page Number:		18596 - 18601
DOI:		10.1073/pnas.0506163102
ISSN/ISBN:		0027-8424 (Print) 1091-6490 (Electronic) 0027-8424 (Linking)
Abstract:		"Many bacterial activities, including expression of virulence factors, horizontal genetic transfer, and production of antibiotics, are controlled by intercellular signaling using small molecules. To date, understanding of the molecular mechanisms of peptide-mediated cell-cell signaling has been limited by a dearth of published information about the molecular structures of the signaling components. Here, we present the molecular structure of PrgX, a DNA- and peptide-binding protein that regulates expression of the conjugative transfer genes of the Enterococcus faecalis plasmid pCF10 in response to an intercellular peptide pheromone signal. Comparison of the structures of PrgX and the PrgX/pheromone complex suggests that pheromone binding destabilizes PrgX tetramers, opening a 70-bp pCF10 DNA loop required for conjugation repression"
Keywords:		"Base Sequence Conjugation, Genetic/*physiology Crystallography, X-Ray DNA, Bacterial/chemistry/metabolism Dimerization Enterococcus faecalis/*chemistry/*physiology Models, Molecular Molecular Sequence Data Nucleic Acid Conformation Protein Binding Protein;"
Notes:		"MedlineShi, Ke Brown, C Kent Gu, Zu-Yi Kozlowicz, Briana K Dunny, Gary M Ohlendorf, Douglas H Earhart, Cathleen A eng R01 AI057585/AI/NIAID NIH HHS/ R01 AI50607/AI/NIAID NIH HHS/ R01 GM049530/GM/NIGMS NIH HHS/ R01 AG050607/AG/NIA NIH HHS/ R01 AI57585/AI/NIAID NIH HHS/ R01 GM49530/GM/NIGMS NIH HHS/ T32 G08347/PHS HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't 2005/12/13 Proc Natl Acad Sci U S A. 2005 Dec 20; 102(51):18596-601. doi: 10.1073/pnas.0506163102. Epub 2005 Dec 8"