| Title: | "Prevalence, phenotype and genotype of oral enterococci" |
| Author(s): | Sedgley CM; Lennan SL; Clewell DB; |
| Address: | "Department of Cariology, Restorative Sciences and Endodontics, University of Michigan School of Dentistry, Ann Arbor, MI 48109-1078, USA. csedgley@umich.edu" |
| DOI: | 10.1111/j.0902-0055.2004.00122.x |
| ISSN/ISBN: | 0902-0055 (Print) 0902-0055 (Linking) |
| Abstract: | "This study investigated the prevalence, phenotype and genotype of oral enterococci. Enterococci were detected in oral rinse samples from 11% of 100 patients receiving endodontic treatment and 1% of 100 dental students with no history of endodontic treatment (P=0.0027). All enterococcal isolates were identified as Enterococcus faecalis. Viable counts ranged from 1 x 10 to 6 x 103 colony forming units per mL of oral rinse sample. Potential virulence traits expressed by oral E. faecalis strains included production of hemolysin (n=4) and gelatinase (n=4), and response to pheromones in E. faecalis culture filtrate (n=1). Six strains produced bacteriocin. All strains were susceptible to ampicillin, benzylpenicillin, gentamicin and vancomycin. There was no evidence of metal-ion resistance. One isolate produced hemolysin, gelatinase and bacteriocin, was resistant to several antibiotics, and responded to the pheromone cPD1. Pulsed-field gel electrophoresis and plasmid analysis showed that oral E. faecalis exhibited widespread genetic polymorphism, with plasmids detected in seven strains" |
| Keywords: | "Adult Aged Anti-Bacterial Agents/pharmacology Bacteriocins/biosynthesis Cohort Studies Colony Count, Microbial Drug Resistance, Bacterial Enterococcus faecalis/*classification/genetics/pathogenicity Female Gelatinases/biosynthesis Genotype Hemolysin Prote;" |
| Notes: | "MedlineSedgley, C M Lennan, S L Clewell, D B eng GM33956/GM/NIGMS NIH HHS/ Research Support, U.S. Gov't, P.H.S. Denmark 2004/02/12 Oral Microbiol Immunol. 2004 Apr; 19(2):95-101. doi: 10.1111/j.0902-0055.2004.00122.x" |
