| Title: | Volatile atmospheric pressure chemical ionisation mass spectrometry headspace analysis of E. coli and S. aureus |
| Author(s): | Rosenthal K; Hunsicker E; Ratcliffe E; Lindley MR; Leonard J; Hitchens JR; Turner MA; |
| Address: | "School of Sport, Exercise & Health Sciences, Loughborough University, Loughborough, UK. m.r.lindley@lboro.ac.uk. Department of Mathematical Sciences, Loughborough University, Loughborough, UK. Department of Chemical Engineering, Loughborough University, Loughborough, UK. Translational Chemical Biology Research Group, Loughborough University, Loughborough, UK. Department of Chemistry, Loughborough University, Loughborough, UK" |
| ISSN/ISBN: | 1759-9679 (Electronic) 1759-9660 (Linking) |
| Abstract: | "Identifying the characteristics of bacterial species can improve treatment outcomes and mass spectrometry methods have been shown to be capable of identifying biomarkers of bacterial species. This study is the first to use volatile atmospheric pressure chemical ionisation mass spectrometry to directly and non-invasively analyse the headspace of E. coli and S. aureus bacterial cultures, enabling major biological classification at species level (Gram negative/positive respectively). Four different protocols were used to collect data, three utilising discrete 5 min samples taken between 2 and 96 h after inoculation and one method employing 24 h continuous sampling. Characteristic marker ions were found for both E. coli and S. aureus. A model to distinguish between sample types was able to correctly identify the bacteria samples after sufficient growth (24-48 h), with similar results obtained across different sampling methods. This demonstrates that this is a robust method to analyse and classify bacterial cultures accurately and within a relevant time frame, offering a promising technique for both clinical and research applications" |
| Keywords: | Atmospheric Pressure Escherichia coli Mass Spectrometry/methods *Methicillin-Resistant Staphylococcus aureus Staphylococcus aureus *Volatile Organic Compounds/analysis/chemistry; |
| Notes: | "MedlineRosenthal, Kerry Hunsicker, Eugenie Ratcliffe, Elizabeth Lindley, Martin R Leonard, Joshua Hitchens, Jack R Turner, Matthew A eng Research Support, Non-U.S. Gov't England 2021/11/16 Anal Methods. 2021 Nov 25; 13(45):5441-5449. doi: 10.1039/d1ay01555a" |
