| Title: | Rapid Collection of Floral Fragrance Volatiles using a Headspace Volatile Collection Technique for GC-MS Thermal Desorption Sampling |
| Author(s): | Ray HA; Stuhl CJ; Gillett-Kaufman JL; |
| Address: | "Entomology and Nematology Department, University of Florida; haleighray12@gmail.com. Center for Medical, Agricultural and Veterinary Entomology, Agricultural Research Service U.S. Department of Agriculture. Entomology and Nematology Department, University of Florida" |
| ISSN/ISBN: | 1940-087X (Electronic) 1940-087X (Linking) |
| Abstract: | "Fragrances of many flower families have been sampled and the volatiles analyzed. Knowing the compounds that make up the fragrances can be an important step to conservation of flowers that are threatened or endangered. Because floral fragrance is critical for attracting pollinators, this method could be used to better understand or even enhance pollination. We present a protocol using a portable charcoal air filter and vacuum to collect floral fragrance volatiles, which are then analyzed by a GC-MS. By using this method, fragrance volatiles can be sampled using a non-destructive method with a machine that is easily transported. This methodology uses a rapid sampling procedure, cutting sampling time down from 2-3 hours to approximately 10 minutes. Using GC-MS, the fragrance compounds can be identified individually, based on authentic standards. The steps used for collecting fragrance and control data are presented, from material setup to collecting the data output" |
| Keywords: | Flowers/*chemistry Gas Chromatography-Mass Spectrometry/*methods Volatile Organic Compounds/*chemistry; |
| Notes: | "MedlineRay, Haleigh A Stuhl, Charles J Gillett-Kaufman, Jennifer L eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Video-Audio Media 2019/12/31 J Vis Exp. 2019 Dec 10; (154). doi: 10.3791/58928" |
