| Title: | Volatile organic compounds obtained by in vitro callus cultivation of Plectranthus ornatus Codd. (Lamiaceae) |
| Author(s): | Passinho-Soares HC; Meira PR; David JP; Mesquita PR; do Vale AE; de MR; de PP; de Santana JR; de Oliveira FS; de Andrade JB; David JM; |
| Address: | "Faculdade de Farmacia, Universidade Federal da Bahia, Rua Barao de Jeremoabo, s/n, 41810-290, Salvador (BA), Brazil. juceni@ufba.br" |
| DOI: | 10.3390/molecules180910320 |
| ISSN/ISBN: | 1420-3049 (Electronic) 1420-3049 (Linking) |
| Abstract: | "Plectranthus spp (Lamiaceae) are plants of economic importance because they are sources of aromatic essential oils and are also cultivated and several species of this genus are used as folk medicines. This paper describes the effects of different concentrations of the 2,4-dichlorophenoxyacetic acid (2,4-D) and 1-naphthaleneacetic acid (NAA) on the induction of callus from nodal segments of Plectranthus ornatus Codd and in the production of volatile organic compounds (monoterpenes and sesquiterpenes). The 20 and 40 day calli were subjected to solid phase micro extraction (HS-SPME) and submitted to GCMS analysis. Variations in VOCs between the samples were observed and, a direct relationship was observed between of the major constituent detected (alpha-terpinyl acetate) and the monoterpenes alpha-thujene, alpha-pinene, beta-pinene, camphene, sabinene and alpha-limonene that were present in the volatile fractions. Besides alpha-terpinyl acetate, isobornyl acetate and alpha-limonene were also major constituents. Variations were observed in VOCs in the analyzed periods. The best cultivation media for the production of VOCs was found to be MS0 (control). Moderate success was achieved by treatment with 2.68 microM and 5:37 microM NAA (Group 2). With 2,4-D (9.0 microM), only the presence of alpha-terpinyl acetate and isocumene were detected and, with 2.26 microM of 2,4-D was produced mainly alpha-terpinyl acetate, alpha-thujene and beta-caryophyllene (16.2%). The VOC profiles present in P. ornatus were interpreted using PCA and HCA. The results permitted us to determine the best cultivation media for VOC production and, the PCA and HCA analysis allowed us to recognize four groups among the different treatments from the compounds identified in this set of treatments" |
| Keywords: | "Gas Chromatography-Mass Spectrometry Monoterpenes/chemistry/*isolation & purification/metabolism Oils, Volatile/chemistry/isolation & purification Plant Components, Aerial/*chemistry/growth & development/metabolism Plant Extracts/chemistry/isolation & pur;" |
| Notes: | "MedlinePassinho-Soares, Helna C Meira, Paloma R David, Juceni P Mesquita, Paulo R R do Vale, Ademir E de M Rodrigues, Frederico de P Pereira, Pedro A de Santana, Jose Raniere F de Oliveira, Fabio S de Andrade, Jailson B David, Jorge M eng Research Support, Non-U.S. Gov't Switzerland 2013/09/26 Molecules. 2013 Aug 26; 18(9):10320-33. doi: 10.3390/molecules180910320" |
