| Title: | Quantitative determination of daumone in rat plasma by liquid chromatography-mass spectrometry |
| Author(s): | Noh K; Park JH; Park JH; Kim M; Jung M; Ha H; Kwon KI; Lee HJ; Kang W; |
| Address: | "College of Pharmacy, Yeungnam University, Kyoungsan, Kyoungbuk 712-749, South Korea" |
| DOI: | 10.1016/j.jpba.2011.04.008 |
| ISSN/ISBN: | 1873-264X (Electronic) 0731-7085 (Linking) |
| Abstract: | "Daumone, 6-(3,5-dihydroxy-6-methyl-tetrahydro-pyran-2-yloxy)-heptanoic acid is a pheromone secreted by Caenorhabditis elegans, and has been known as a pivotal regulator of chemosensory processes in development and ageing. A quantification method using mass spectrometry was developed for the determination of daumone in rat plasma. After simple protein precipitation with acetonitrile including an internal standard, the analytes were chromatographed on a reversed-phase column and detected by liquid chromatography/tandem mass spectrometry with electrospray ionization. The accuracy and precision of the assay were in accordance with FDA regulations for validation of bioanalytical methods. This method was applied to measure the plasma daumone concentrations after a single intravenous administration of daumone in rats" |
| Keywords: | "Animals Calibration Chromatography, Liquid/*methods Fatty Acids/*blood/pharmacokinetics Limit of Detection Male Pheromones/*blood/pharmacokinetics Rats Reference Standards Reproducibility of Results Spectrometry, Mass, Electrospray Ionization/*methods Tan;" |
| Notes: | "MedlineNoh, Keumhan Park, Jung Hyun Park, Jong Hee Kim, Minkyu Jung, Mankil Ha, Hunjoo Kwon, Kwang-Il Lee, Hwa Jeong Kang, Wonku eng Research Support, Non-U.S. Gov't England 2011/05/24 J Pharm Biomed Anal. 2011 Aug 25; 56(1):114-7. doi: 10.1016/j.jpba.2011.04.008. Epub 2011 Apr 15" |
