« Previous Abstract"Understanding and exploring the potentials of household water treatment methods for volatile disinfection by-products control: Kinetics, mechanisms, and influencing factors"    Next Abstract"Production, identification, and field evaluation of sex pheromone from calling females in Diaphania angustalis (Lepidoptera: Crambidae)" »

J Agric Food Chem

Title:		Two Odorant-Binding Proteins Involved in the Recognition of Sex Pheromones in Spodoptera litura Larvae
Author(s):		Ma S; Li LL; Yao WC; Yin MZ; Li JQ; Xu JW; Dewer Y; Zhu XY; Zhang YN;
Address:		"Anhui Province Key Laboratory of Pollutant Sensitive Materials and Environmental Remediation, College of Life Sciences, Huaibei Normal University, 100 Dongshan Road, Huaibei 235000, China. Institute of Plant Protection, Suzhou Academy of Agricultural Sciences, Suzhou 234000, China. Phytotoxicity Research Department, Central Agricultural Pesticide Laboratory, Agricultural Research Center, 7 Nadi El-Seid Street, Dokki 12618, Giza, Egypt"
Journal Title:		J Agric Food Chem
Year:		2022
Volume:		20220921
Issue:		39
Page Number:		12372 - 12382
DOI:		10.1021/acs.jafc.2c04335
ISSN/ISBN:		1520-5118 (Electronic) 0021-8561 (Linking)
Abstract:		"Usually, the recognition of sex pheromone signals is restricted to adult moths. Here, our behavioral assay showed that fourth-instar Spodoptera litura larvae are attracted to cabbage laced with minor sex pheromones Z9,E12-tetradecadienyl acetate (Z9,E12-14:Ac) or Z9-tetradecenyl acetate (Z9-14:Ac). Seven odorant-binding proteins (OBPs) were upregulated after exposure to Z9,E12-14:Ac, and one OBP was upregulated after exposure to Z9-14:Ac. Fluorescence competitive binding assays showed that GOBP2 and OBP7 bound to sex pheromones. RNAi treatment significantly downregulated GOBP2 and OBP7 mRNA expression by 70.37 and 63.27%, respectively. The siOBP-treated larvae were not attracted to Z9,E12-14:Ac or Z9-14:Ac, and the corresponding preference indices were significantly lower than those in siGFP-treated larvae. Therefore, we concluded that GOBP2 and OBP7 are involved in the attraction of S. litura larvae to food containing Z9,E12-14:Ac and Z9-14:Ac. These results provide an important basis for exploring the olfactory mechanisms underlying sex pheromone attraction in moth larvae"
Keywords:		"Animals Larva/genetics/metabolism *Moths/genetics Odorants Pheromones/metabolism RNA, Messenger/metabolism *Sex Attractants/metabolism/pharmacology Spodoptera/genetics/metabolism Spodoptera litura host plants larvae behavior odorant-binding protein sex ph;"
Notes:		"MedlineMa, Sai Li, Lu Lu Yao, Wei-Chen Yin, Mao-Zhu Li, Jian-Qiao Xu, Ji-Wei Dewer, Youssef Zhu, Xiu-Yun Zhang, Ya-Nan eng 2022/09/22 J Agric Food Chem. 2022 Oct 5; 70(39):12372-12382. doi: 10.1021/acs.jafc.2c04335. Epub 2022 Sep 21"