| Title: | Quantitative iTRAQ proteome and comparative transcriptome analysis of elicitor-induced Norway spruce (Picea abies) cells reveals elements of calcium signaling in the early conifer defense response |
| Author(s): | Lippert DN; Ralph SG; Phillips M; White R; Smith D; Hardie D; Gershenzon J; Ritland K; Borchers CH; Bohlmann J; |
| Address: | "Michael Smith Laboratories, University of British Columbia, Vancouver, BC, Canada" |
| ISSN/ISBN: | 1615-9861 (Electronic) 1615-9853 (Linking) |
| Abstract: | "Long-lived conifer trees depend on both constitutive and induced defenses for resistance against a myriad of potential pathogens and herbivores. In species of spruce (Picea spp.), several of the late events of pathogen-, insect-, or elicitor-induced defense responses have previously been characterized at the anatomical, biochemical, transcriptome, and proteome levels in stems and needles. However, accurately measuring the early events of induced cellular responses in a conifer is technically challenging due to limitations in the precise timing of induction and tissue sampling from intact trees following insect or fungal treatment. In the present study, we used the advantages of Norway spruce (Picea abies) cell suspensions combined with chitosan elicitation to investigate the early proteome response in a conifer. A combination of iTRAQ labeling and a new design of iterative sample analysis employing data-dependent exclusion lists were used for proteome analysis. This approach improved the coverage of the spruce proteome beyond that achieved in any prior study in a conifer system. Comparison of elicitor-induced proteome and transcriptome responses in Norway spruce cells consistently identified features associated with calcium-mediated signaling and response to oxidative stress that have not previously been observed in the response of intact trees to fungal attack" |
| Keywords: | "Calcium Signaling/*genetics/physiology Cell Culture Techniques Chitosan/pharmacology Data Interpretation, Statistical Gene Expression Profiling Gene Expression Regulation, Plant/drug effects/physiology Oligonucleotide Array Sequence Analysis Oxidative Str;" |
| Notes: | "MedlineLippert, Dustin N Ralph, Steven G Phillips, Michael White, Rick Smith, Derek Hardie, Darryl Gershenzon, Jonathan Ritland, Kermit Borchers, Christoph H Bohlmann, Jorg eng Research Support, Non-U.S. Gov't Germany 2008/12/24 Proteomics. 2009 Jan; 9(2):350-67. doi: 10.1002/pmic.200800252" |
