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Cell Chem Biol

Title:		PhoDAGs Enable Optical Control of Diacylglycerol-Sensitive Transient Receptor Potential Channels
Author(s):		Leinders-Zufall T; Storch U; Bleymehl K; Mederos YS; Frank JA; Konrad DB; Trauner D; Gudermann T; Zufall F;
Address:		"Center for Integrative Physiology and Molecular Medicine, Saarland University, 66421 Homburg, Germany. Walther-Straub-Institut fur Pharmakologie und Toxikologie, Ludwig-Maximilians-Universitat Munchen, 80336 Munchen, Germany. Department of Chemistry, Ludwig-Maximilians-Universitat Munchen, 81377 Munchen, Germany. Department of Chemistry, Ludwig-Maximilians-Universitat Munchen, 81377 Munchen, Germany; Department of Chemistry, New York University, New York, NY 10003, USA. Center for Integrative Physiology and Molecular Medicine, Saarland University, 66421 Homburg, Germany. Electronic address: frank.zufall@uks.eu"
Journal Title:		Cell Chem Biol
Year:		2018
Volume:		20171221
Issue:		2
Page Number:		215 - 223
DOI:		10.1016/j.chembiol.2017.11.008
ISSN/ISBN:		2451-9448 (Electronic) 2451-9448 (Linking)
Abstract:		"Diacylglycerol-sensitive transient receptor potential (TRP) channels play crucial roles in a wide variety of biological processes and systems, but their activation mechanism is not well understood. We describe an optical toolkit by which activation and deactivation of these ion channels can be controlled with unprecedented speed and precision through light stimuli. We show that the photoswitchable diacylglycerols PhoDAG-1 and PhoDAG-3 enable rapid photoactivation of two DAG-sensitive TRP channels, Trpc2 and TRPC6, upon stimulation with UV-A light, whereas exposure to blue light terminates channel activation. PhoDAG photoconversion can be applied in heterologous expression systems, in native cells, and even in mammalian tissue slices. Combined laser scanning-controlled photoswitching and Ca(2+) imaging enables both large-scale mapping of TRP channel-mediated neuronal activation and localized mapping in small cellular compartments. Light-switchable PhoDAGs provide an important advance to explore the pathophysiological relevance of DAG-sensitive TRP channels in the maintenance of body homeostasis"
Keywords:		"Animals Diglycerides/chemistry/*metabolism Humans Mice Mice, Inbred C57BL Molecular Structure Photochemical Processes Transient Receptor Potential Channels/chemistry/*metabolism cardiopulmonary function kidney disease lipid signaling olfactory oxygen sens;"
Notes:		"MedlineLeinders-Zufall, Trese Storch, Ursula Bleymehl, Katherin Mederos Y Schnitzler, Michael Frank, James A Konrad, David B Trauner, Dirk Gudermann, Thomas Zufall, Frank eng Research Support, Non-U.S. Gov't 2017/12/26 Cell Chem Biol. 2018 Feb 15; 25(2):215-223.e3. doi: 10.1016/j.chembiol.2017.11.008. Epub 2017 Dec 21"