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Peptides

Title:		"Responsiveness of vomeronasal cells to a newt peptide pheromone, sodefrin as monitored by changes of intracellular calcium concentrations"
Author(s):		Iwata T; Nakada T; Toyoda F; Yada T; Shioda S; Kikuyama S;
Address:		"Department of Biology, Faculty of Education and Integrated Sciences, Waseda University, Tokyo 169-8050, Japan"
Journal Title:		Peptides
Year:		2013
Volume:		20130422
Issue:
Page Number:		15 - 21
DOI:		10.1016/j.peptides.2013.04.006
ISSN/ISBN:		1873-5169 (Electronic) 0196-9781 (Linking)
Abstract:		"A peptide pheromone of the red-bellied male newt, sodefrin was tested for its ability to increase intracellular concentrations of Ca(2+) ([Ca(2+)]i) in the dissociated vomeronasal (VN) cells of females by means of calcium imaging system. The pheromone elicited a marked elevation of [Ca(2+)]i in a small population of VN cells from sexually developed females. The population of cells exhibiting sodefrin-induced elevation of [Ca(2+)]i increased concentration-dependently. A pheromone of a different species was ineffective in this respect. The VN cells from non-reproductive females or from reproductive males scarcely responded to sodefrin in terms of elevating [Ca(2+)]i. In the cells from hypophysectomized and ovariectomized females, the sodefrin-inducible increase of [Ca(2+)]i never occurred. The cells from the operated newts supplemented with prolactin and estradiol exhibited [Ca(2+)]i responses to sodefrin with a high incidence. Thus, sex- and hormone-dependency as well as species-specificity of the responsiveness of the VN cells to sodefrin was evidenced at the cellular level. Subsequently, possibility of involvement of phospholipase C (PLC)-inositol 1,4,5-trisphosphate (IP3) and/or PLC-diacylglycerol (DAG)-protein kinase C (PKC) pathways in increasing [Ca(2+)]i in VN cells in response to sodefrin was explored using pharmacological approaches. The results indicated that PLC is involved in generating the Ca(2+) signal in all sodefrin-responsive VN cells, whereas IP3 in approximately 50% of the cells and DAG-PKC in the remaining cells. In the latter case, the increase of [Ca(2+)]i was postulated to be induced by the influx of Ca(2+) through the L-type channel. The significance of the finding is discussed"
Keywords:		"Animals Calcium/*metabolism Calcium Signaling Cell Proliferation Diglycerides/metabolism Epithelial Cells/cytology/*drug effects/metabolism Estradiol/pharmacology Female Hypophysectomy Inositol 1, 4, 5-Trisphosphate/metabolism Male Molecular Imaging Oligope;"
Notes:		"MedlineIwata, Takeo Nakada, Tomoaki Toyoda, Fumiyo Yada, Toshihiko Shioda, Seiji Kikuyama, Sakae eng Research Support, Non-U.S. Gov't 2013/04/27 Peptides. 2013 Jul; 45:15-21. doi: 10.1016/j.peptides.2013.04.006. Epub 2013 Apr 22"