| Title: | Spindle-independent condensation-mediated segregation of yeast ribosomal DNA in late anaphase |
| Author(s): | Machin F; Torres-Rosell J; Jarmuz A; Aragon L; |
| Address: | "Cell Cycle Group, Clinical Sciences Centre, Medical Research Council, Imperial College London, London W12 0NN, England, UK" |
| ISSN/ISBN: | 0021-9525 (Print) 1540-8140 (Electronic) 0021-9525 (Linking) |
| Abstract: | "Mitotic cell division involves the equal segregation of all chromosomes during anaphase. The presence of ribosomal DNA (rDNA) repeats on the right arm of chromosome XII makes it the longest in the budding yeast genome. Previously, we identified a stage during yeast anaphase when rDNA is stretched across the mother and daughter cells. Here, we show that resolution of sister rDNAs is achieved by unzipping of the locus from its centromere-proximal to centromere-distal regions. We then demonstrate that during this stretched stage sister rDNA arrays are neither compacted nor segregated despite being largely resolved from each other. Surprisingly, we find that rDNA segregation after this period no longer requires spindles but instead involves Cdc14-dependent rDNA axial compaction. These results demonstrate that chromosome resolution is not simply a consequence of compacting chromosome arms and that overall rDNA compaction is necessary to mediate the segregation of the long arm of chromosome XII" |
| Keywords: | "Anaphase/*physiology Bacterial Proteins/genetics Carrier Proteins/genetics Cell Cycle/drug effects Cell Cycle Proteins/genetics/metabolism/physiology Chromosome Segregation/*physiology DNA, Ribosomal/genetics/*metabolism DNA-Binding Proteins/genetics/meta;" |
| Notes: | "MedlineMachin, Felix Torres-Rosell, Jordi Jarmuz, Adam Aragon, Luis eng MC_U120074328/MRC_/Medical Research Council/United Kingdom Research Support, Non-U.S. Gov't 2005/01/20 J Cell Biol. 2005 Jan 17; 168(2):209-19. doi: 10.1083/jcb.200408087" |
