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Methods Enzymol

Title:		Structure of odorant binding proteins and chemosensory proteins determined by X-ray crystallography
Author(s):		Gaubert A; Amigues B; Spinelli S; Cambillau C;
Address:		"Architecture et Fonction des Macromolecules Biologiques, Centre National de la Recherche Scientifique (CNRS) & Aix-Marseille Universite, Marseille, France. Architecture et Fonction des Macromolecules Biologiques, Centre National de la Recherche Scientifique (CNRS) & Aix-Marseille Universite, Marseille, France. Electronic address: cambillau@afmb.univ-mrs.fr"
Journal Title:		Methods Enzymol
Year:		2020
Volume:		20200527
Issue:
Page Number:		151 - 167
DOI:		10.1016/bs.mie.2020.04.070
ISSN/ISBN:		1557-7988 (Electronic) 0076-6879 (Linking)
Abstract:		"OBPs and CSPs are small soluble proteins used by organisms as shuttle to transport odorant molecules between air and the membrane-embedded receptors. Deciphering the interactions of these proteins with their ligands at a molecular level may give clue on the function and specificity of the olfactory chain. To reach this goal, protein crystallography is very helpful with more than hundred entries available in the protein data bank (PDB). In this chapter, we present the peculiarities of OBPs and CSPs concerning their crystallization and 3D structure determination by X-ray diffraction"
Keywords:		"Carrier Proteins Crystallography, X-Ray Insect Proteins/metabolism *Odorants Phylogeny *Receptors, Odorant/genetics/metabolism Chemosensory proteins Crystallization Odorant binding proteins Pheromone binding proteins X-ray diffraction;"
Notes:		"MedlineGaubert, Anais Amigues, Beatrice Spinelli, Silvia Cambillau, Christian eng 2020/08/24 Methods Enzymol. 2020; 642:151-167. doi: 10.1016/bs.mie.2020.04.070. Epub 2020 May 27"