| Title: | Multisite imaging of neural activity using a genetically encoded calcium sensor in the honey bee |
| Author(s): | Carcaud J; Otte M; Grunewald B; Haase A; Sandoz JC; Beye M; |
| Address: | "Evolution, Genomes, Behavior and Ecology, Universite Paris-Saclay, CNRS, IRD, Gif-sur-Yvette, France. Evolutionnary Genetics, Heinrich Heine University Dusseldorf, Dusseldorf, Germany. Institut fur Bienenkunde, Polytechnische Gesellschaft, FB Biowissenschaften, Goethe-University, Frankfurt am Main, Germany. Center for Mind/Brain Sciences (CIMeC), University of Trento, Rovereto, Italy. Department of Physics, University of Trento, Trento, Italy" |
| DOI: | 10.1371/journal.pbio.3001984 |
| ISSN/ISBN: | 1545-7885 (Electronic) 1544-9173 (Print) 1544-9173 (Linking) |
| Abstract: | "Understanding of the neural bases for complex behaviors in Hymenoptera insect species has been limited by a lack of tools that allow measuring neuronal activity simultaneously in different brain regions. Here, we developed the first pan-neuronal genetic driver in a Hymenopteran model organism, the honey bee, and expressed the calcium indicator GCaMP6f under the control of the honey bee synapsin promoter. We show that GCaMP6f is widely expressed in the honey bee brain, allowing to record neural activity from multiple brain regions. To assess the power of this tool, we focused on the olfactory system, recording simultaneous responses from the antennal lobe, and from the more poorly investigated lateral horn (LH) and mushroom body (MB) calyces. Neural responses to 16 distinct odorants demonstrate that odorant quality (chemical structure) and quantity are faithfully encoded in the honey bee antennal lobe. In contrast, odor coding in the LH departs from this simple physico-chemical coding, supporting the role of this structure in coding the biological value of odorants. We further demonstrate robust neural responses to several bee pheromone odorants, key drivers of social behavior, in the LH. Combined, these brain recordings represent the first use of a neurogenetic tool for recording large-scale neural activity in a eusocial insect and will be of utility in assessing the neural underpinnings of olfactory and other sensory modalities and of social behaviors and cognitive abilities" |
| Keywords: | Bees/genetics Animals *Calcium *Smell/genetics Odorants Brain/physiology Pheromones/genetics; |
| Notes: | "MedlineCarcaud, Julie Otte, Marianne Grunewald, Bernd Haase, Albrecht Sandoz, Jean-Christophe Beye, Martin eng Research Support, Non-U.S. Gov't 2023/02/01 PLoS Biol. 2023 Jan 31; 21(1):e3001984. doi: 10.1371/journal.pbio.3001984. eCollection 2023 Jan" |
