« Previous AbstractReactive Uptake of Dimethylamine by Ammonium Sulfate and Ammonium Sulfate-Sucrose Mixed Particles    Next AbstractReduction in VOC emissions by intermittent aeration in bioreactor landfills with gas-water joint regulation »

Anal Bioanal Chem

Title:		Variable VOCs in plastic culture flasks and their potential impact on cell volatile biomarkers
Author(s):		Chu Y; Zhou J; Ge D; Lu Y; Zou X; Xia L; Huang C; Shen C; Chu Y;
Address:		"Anhui Province Key Laboratory of Medical Physics and Technology, Institute of Health & Medical Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, 230031, Anhui, China. Institute of Physical Science and Information Technology, Anhui University, Hefei, 230601, Anhui, China. University of Science and Technology of China, Hefei, 230026, Anhui, China. Hefei Cancer Hospital, Chinese Academy of Sciences, Hefei, 230031, Anhui, China. Anhui Province Key Laboratory of Medical Physics and Technology, Institute of Health & Medical Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, 230031, Anhui, China. ychu@aiofm.ac.cn"
Journal Title:		Anal Bioanal Chem
Year:		2020
Volume:		20200620
Issue:		22
Page Number:		5397 - 5408
DOI:		10.1007/s00216-020-02756-9
ISSN/ISBN:		1618-2650 (Electronic) 1618-2642 (Linking)
Abstract:		"In order to find out cancer markers in human breath, in vitro cell culture is often used to study the characteristic volatile organic compounds (VOCs). In the cell culture process, disposable vessels are frequently adopted. However, these vessels are normally made of plastic, and they have the possibility to release some VOCs, which may interfere with the cell-specific volatiles and even can result in an incorrect conclusion. In this study, by using glass cell culture flasks as control, the headspace solid-phase microextraction gas chromatography mass spectrometry (HS-SPME-GC-MS) analyses of the VOCs in plastic cell culture flasks were systematically carried out for the first time. A total of 35 VOCs were detected in five brands of flasks. In each flask, there were between 13 and 25 volatile compounds. Furthermore, the components and packaging bag of each flask were also sampled and analyzed by HS-SPME-GC-MS. The results show that the flask cap, septum, flask body, and packaging bag exhibit respectively different volatile behaviors. The former two parts release the most volatiles which have obvious contributions to the headspace gases in the flasks, while the flask body mainly liberates styrene. For different flasks packed within the same bag, the headspace analyses show that their residual VOCs are inconsistent with each other. Moreover, the residual VOCs in the same flask are variable in three consecutive days. These results indicate that the multiple flasks in parallel cell culture experiments, or the same flask with different cell culture durations, will produce an indelible disturbance to the cell-specific VOCs. In addition, among the 35 VOCs detectable in five brands of empty plastic flasks, 15 VOCs were previously reported as characteristic VOCs from lung cancer, melanoma, cervical cancer cells, or normal cells. This is an alert that, when using plastic flasks, it must be careful to treat the possible interference from the background VOCs in the flasks. This study demonstrates that the cell culture tool needs to be standardized, and the clean glass or metal vessels are strongly recommended for usage when studying cell volatile biomarkers. Graphical abstract"
Keywords:		"Biomarkers, Tumor/*analysis Breath Tests Cell Culture Techniques Gas Chromatography-Mass Spectrometry/methods Humans Neoplasms/diagnosis *Plastics Solid Phase Microextraction/methods Volatile Organic Compounds/*analysis Biomarker Cancer cell Culture flask;"
Notes:		"MedlineChu, Yajing Zhou, Jijuan Ge, Dianlong Lu, Yan Zou, Xue Xia, Lei Huang, Chaoqun Shen, Chengyin Chu, Yannan eng 21876176/National Natural Science Foundation of China/ Germany 2020/06/22 Anal Bioanal Chem. 2020 Sep; 412(22):5397-5408. doi: 10.1007/s00216-020-02756-9. Epub 2020 Jun 20"