« Previous AbstractRedox regulation of the yeast voltage-gated Ca(2+) channel homolog Cch1p by glutathionylation of specific cysteine residues    Next AbstractA paracrine peptide sex pheromone also acts as an autocrine signal to induce plasmid transfer and virulence factor expression in vivo »

Probiotics Antimicrob Proteins

Title:		Synergistic Effect of Biogenics Derived from Potential Probiotics Together with Zingerone Against Biofilm Formation by Pseudomonas aeruginosa PAO1
Author(s):		Chandla S; Harjai K; Shukla G;
Address:		"Department of Microbiology, Panjab University, Basic Medical Sciences (Block-1), South Campus Sector-25, 160014, Chandigarh, India. Department of Microbiology, Panjab University, Basic Medical Sciences (Block-1), South Campus Sector-25, 160014, Chandigarh, India. geeta_shukla@pu.ac.in"
Journal Title:		Probiotics Antimicrob Proteins
Year:		2021
Volume:		20210330
Issue:		5
Page Number:		1481 - 1497
DOI:		10.1007/s12602-021-09763-x
ISSN/ISBN:		1867-1314 (Electronic) 1867-1306 (Linking)
Abstract:		"Biogenics are compounds produced by living organisms such as animals, plants, bacteria, etc. Probiotics and their biogenics are known for their antimicrobial potential. Therefore, the present study was designed to evaluate the antibiofilm potential of probiotic-derived biogenics in conjunction with zingerone against the Pseudomonas aeruginosa PAO1 biofilm. Cell-free supernatant (CFS) of potential probiotics Pediococcus acidilactici BNS5B and Lactobacillus fermentum PUM was found to inhibit the growth of Ps. aeruginosa PAO1 maximally among the nineteen isolated lactic acid bacteria. L. fermentum PUM produced precipitated protein fraction (PP), organic acids (OAs), exopolysaccharides (EPSs), biosurfactants (BSs) and various volatile antimicrobial compounds, while Ped. acidilactici BNS5B was found to produce PP, OA, EPS, BS and fewer volatile antimicrobial compounds only. More specifically, CFS and selected biogenics (OA and PP from L. fermentum PUM; OA from Ped. acidilactici BNS5B) of both potential probiotics showed synergy with zingerone against Ps. aeruginosa growth as observed by FIC index (< 0.5). Interestingly, CFS of both potential probiotics in combination with zingerone led to the formation of a more distorted biofilm compared with OA of L. fermentum PUM and zingerone, OA of Ped. acidilactici BNS5B and zingerone, PP of L. fermentum PUM and zingerone as well as their individual counterparts. Similarly, both confocal laser scanning microscopy and XTT assay showed an increased number of dead and impaired cells along with the decreased viability of biofilm cells. Thus, it can be reckoned that a combination of probiotic-derived biogenics and zingerone can have therapeutic application against Ps. aeruginosa infections which needs to be validated clinically"
Keywords:		Animals;Anti-Infective Agents/*pharmacology Biofilms/drug effects Biological Products/*pharmacology Guaiacol/*analogs & derivatives/pharmacology *Probiotics Pseudomonas aeruginosa/*drug effects Biofilm Biogenics Cell free supernatant Probiotics Zingerone;
Notes:		"MedlineChandla, Swati Harjai, Kusum Shukla, Geeta eng Research Support, Non-U.S. Gov't 2021/03/31 Probiotics Antimicrob Proteins. 2021 Oct; 13(5):1481-1497. doi: 10.1007/s12602-021-09763-x. Epub 2021 Mar 30"