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Diagn Microbiol Infect Dis

Title:		The rapid evaluation of bacterial growth and antibiotic susceptibility in blood cultures by selected ion flow tube mass spectrometry
Author(s):		Allardyce RA; Hill AL; Murdoch DR;
Address:		"Department of Surgery, Christchurch School of Medicine and Health Sciences, PO Box 4345, Christchurch, New Zealand. randall.allardyce@chmeds.ac.nz"
Journal Title:		Diagn Microbiol Infect Dis
Year:		2006
Volume:		20060504
Issue:		4
Page Number:		255 - 261
DOI:		10.1016/j.diagmicrobio.2006.01.031
ISSN/ISBN:		0732-8893 (Print) 0732-8893 (Linking)
Abstract:		"We have measured the production of volatile organic compounds (VOCs) by selected ion flow tube mass spectrometry (SIFT-MS) in the headspaces of conventional BacT/ALERT blood culture bottles (Biomerieux, Durham, NC) artificially infected with 5 bacterial strains. Uninfected blood samples were inoculated with Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus pneumoniae, Escherichia coli, and Neisseria meningitidis. Growth and species identification were determined at 6 h by measuring a panel of 9 VOC products. Two species, E. coli and S. aureus, were also incubated in the presence of gentamicin or flucloxacillin, respectively, above or below their demonstrated MIC. The concentration-dependent antibiotic susceptibility of both strains was demonstrated by the inhibition of VOC production at 22 h (P < .05). These results suggest incorporating SIFT-MS detection of microbial VOCs as a sensitive method for bacterial detection, identification, and determination of antibiotic susceptibility in a conventional blood culture system"
Keywords:		Bacteremia/diagnosis/*microbiology Bacterial Typing Techniques/*methods Escherichia coli/drug effects/isolation & purification/metabolism Gas Chromatography-Mass Spectrometry/instrumentation Gram-Positive Cocci/drug effects/isolation & purification/metabo;
Notes:		"MedlineAllardyce, Randall A Hill, Alex L Murdoch, David R eng Research Support, Non-U.S. Gov't 2006/05/09 Diagn Microbiol Infect Dis. 2006 Aug; 55(4):255-61. doi: 10.1016/j.diagmicrobio.2006.01.031. Epub 2006 May 4"