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Gene

Title:		Antenna-predominant and male-biased CSP19 of Sesamia inferens is able to bind the female sex pheromones and host plant volatiles
Author(s):		Zhang YN; Ye ZF; Yang K; Dong SL;
Address:		"Education Ministry Key Laboratory of Integrated Management of Crop Diseases and Pests, Nanjing, China; College of Plant Protection, Nanjing Agricultural University, Nanjing, China. Education Ministry Key Laboratory of Integrated Management of Crop Diseases and Pests, Nanjing, China; College of Plant Protection, Nanjing Agricultural University, Nanjing, China. Electronic address: sldong@njau.edu.cn"
Journal Title:		Gene
Year:		2014
Volume:		20131218
Issue:		2
Page Number:		279 - 286
DOI:		10.1016/j.gene.2013.12.011
ISSN/ISBN:		1879-0038 (Electronic) 0378-1119 (Linking)
Abstract:		"Insect chemosensory proteins (CSPs) are proposed to capture and transport hydrophobic chemicals across the sensillum lymph to olfactory receptors (ORs), but this has not been clarified in moths. In this study, we built on our previously reported segment sequence work and cloned the full length CSP19 gene (SinfCSP19) from the antennae of Sesamia inferens by using rapid amplification of cDNA ends. Quantitative real time-PCR (qPCR) assays indicated that the gene was expressed in a unique profile, i.e. predominant in antennae and significantly higher in male than in female. To explore the function, recombinant SinfCSP19 was expressed in Escherichia coli cells and purified by Ni-ion affinity chromatography. Binding affinities of the recombinant SinfCSP19 with 39 plant volatiles, 3 sex pheromone components and 10 pheromone analogs were measured using fluorescent competitive binding assays. The results showed that 6 plant volatiles displayed high binding affinities to SinfCSP19 (Ki = 2.12-8.75 muM), and more interesting, the 3 sex pheromone components and analogs showed even higher binding to SinfCSP19 (Ki = 0.49-1.78 muM). Those results suggest that SinfCSP19 plays a role in reception of female sex pheromones of S. inferens and host plant volatiles"
Keywords:		"Amino Acid Sequence Animals Arthropod Antennae/*metabolism Binding, Competitive/*genetics DNA, Complementary/genetics Female Insect Proteins/genetics/*metabolism Male Molecular Sequence Data Moths/genetics/*metabolism Phylogeny Protein Binding/*genetics R;"
Notes:		"MedlineZhang, Ya-Nan Ye, Zhan-Feng Yang, Ke Dong, Shuang-Lin eng Research Support, Non-U.S. Gov't Netherlands 2013/12/24 Gene. 2014 Feb 25; 536(2):279-86. doi: 10.1016/j.gene.2013.12.011. Epub 2013 Dec 18"