| Title: | Dual functions of CDC6: a yeast protein required for DNA replication also inhibits nuclear division |
| Address: | "Department of Molecular and Cell Biology, Scripps Research Institute, La Jolla, CA 92037" |
| DOI: | 10.1002/j.1460-2075.1992.tb05276.x |
| ISSN/ISBN: | 0261-4189 (Print) 1460-2075 (Electronic) 0261-4189 (Linking) |
| Abstract: | "The Saccharomyces cerevisiae gene CDC6, whose protein product is required for DNA replication, is transcribed only in late G1 and S phases. We have discovered a critical reason why CDC6 expression is regulated in this fashion. Constitutive CDC6 transcription greatly delayed the initiation of M phase without effecting the G1-S transition or growth rate. This occurred in both fission and budding yeasts. The CDC6-induced M phase delay was dependent on the wee1/mik1 mitotic inhibitor kinases and was greatly accentuated in strains defective for the cdc25/MIH1 mitotic inducer phosphatases, indicating that CDC6 indirectly inhibits activation of the p34cdc2/CDC28 M phase kinase. Thus CDC6 appears to have an important and perhaps unique dual role in S phase, it is first required for the initiation of DNA replication and then actively participates in the suppression of nuclear division" |
| Keywords: | "Base Sequence Cell Cycle/drug effects/*genetics *Cell Cycle Proteins Cell Division Cell Nucleus/*physiology/ultrastructure DNA Replication/*genetics Fungal Proteins/*genetics/*metabolism *Genes, Fungal Mating Factor Mitosis/genetics Molecular Sequence Dat;" |
| Notes: | "MedlineBueno, A Russell, P eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. England 1992/06/01 EMBO J. 1992 Jun; 11(6):2167-76. doi: 10.1002/j.1460-2075.1992.tb05276.x" |
