« Previous Abstract"Aphid-repellent, ladybug-attraction activities, and binding mechanism of methyl salicylate derivatives containing geraniol moiety"    Next AbstractInhibition of enteric methanogenesis in dairy cows induces changes in plasma metabolome highlighting metabolic shifts and potential markers of emission »

Pak J Pharm Sci

Title:		GC-MS analyses of the volatiles of Houttuynia cordata Thunb
Author(s):		Yang ZN; Luo SQ; Ma J; Wu D; Hong L; Yu ZW;
Address:		"Key Laboratory for Information System of Mountainous Area and Protection of Ecological Environment of Guizhou Province, Guizhou Normal University, Guiyang, PR China. School of Life Science, Guizhou Normal University, Guiyang, PR China"
Journal Title:		Pak J Pharm Sci
Year:		2016
Volume:		29
Issue:		5
Page Number:		1591 - 1600
DOI:
ISSN/ISBN:		1011-601X (Print) 1011-601X (Linking)
Abstract:		"GC-MS is the basis of analysis of plant volatiles. Several protocols employed for the assay have resulted in inconsistent results in the literature. We developed a GC-MS method, which were applied to analyze 25 volatiles (alpha-pinene, camphene, beta-pinene, 2-methyl-2-pentenal, myrcene, (+)-limonene, eucalyptol, trans-2-hexenal, gamma-terpinene, cis-3-hexeneyl-acetate, 1-hexanol, alpha-pinene oxide, cis-3-hexen-1-ol, trans-2-hexen-1-ol, decanal, linalool, acetyl-borneol, beta-caryophyllene, 2-undecanone, 4-terpineol, borneol, decanol, eugenol, isophytol and phytol) of Houttuynia cordata Thunb. Linear behaviors for all analytes were observed with a linear regression relationship (r2>0.9991) at the concentrations tested. Recoveries of the 25 analytes were 98.56-103.77% with RSDs <3.0%. Solution extraction (SE), which involved addition of an internal standard, could avoid errors for factors in sample preparation by steam distillation (SD) and solidphase micro extraction (SPME). Less sample material ( asymptotically equal to0.05g fresh leaves of H. cordata) could be used to determine the contents of 25 analytes by our proposed method and, after collection, did not affect the normal physiological activity or growth of H. cordata. This method can be used to monitor the metabolic accumulation of H. cordata volatiles"
Keywords:		"Calibration *Gas Chromatography-Mass Spectrometry/standards Houttuynia/*chemistry Linear Models Phytotherapy Plant Extracts/*isolation & purification Plant Leaves Plants, Medicinal Reference Standards Reproducibility of Results Volatile Organic Compounds/;"
Notes:		"MedlineYang, Zhan-Nan Luo, Shi-Qiong Ma, Jing Wu, Dan Hong, Liang Yu, Zheng-Wen eng Validation Study Pakistan 2016/10/13 Pak J Pharm Sci. 2016 Sep; 29(5):1591-1600"