| Title: | Monopolar spindle attachment of sister chromatids is ensured by two distinct mechanisms at the first meiotic division in fission yeast |
| Address: | "CREST Research Project, Kansai Advanced Research Center, Communications Research Laboratory, 588-2 Iwaoka, Iwaoka-cho, Nishi-ku, Kobe 651-2492, Japan. ayumu@crl.go.jp" |
| ISSN/ISBN: | 0261-4189 (Print) 1460-2075 (Electronic) 0261-4189 (Linking) |
| Abstract: | "At meiosis I, sister chromatids attach to the same spindle pole (i.e. monopolar attachment). Mechanisms establishing monopolar attachment remain largely unknown. In the fission yeast Schizosaccharomyces pombe, monopolar attachment is established in haploid cells, indicating that homologous chromosomes are dispensable for its establishment. This monopolar attachment requires both mating pheromone signaling and inactivation of Pat1 kinase (a key negative regulator of meiosis). It also requires the meiotic cohesin factor Rec8 but not the recombination factor Rec12. In contrast, in diploid cells, monopolar attachment is established by Pat1 inactivation alone, and does not require mating pheromone signaling. Furthermore, monopolar attachment requires Rec12 in addition to Rec8. These results indicate that monopolar attachment of sister chromatids can be established by two distinct mechanisms in S.pombe, one that is pheromone dependent and recombination independent, and a second that is pheromone independent and recombination dependent. We propose that co-operation of these two mechanisms generates the high fidelity of monopolar attachment" |
| Keywords: | "Base Sequence Chromatids/*physiology DNA Primers Fluorescence Meiosis/*physiology Recombination, Genetic Schizosaccharomyces/*physiology Schizosaccharomyces pombe Proteins/physiology;" |
| Notes: | "MedlineYamamoto, Ayumu Hiraoka, Yasushi eng Research Support, Non-U.S. Gov't England 2003/05/03 EMBO J. 2003 May 1; 22(9):2284-96. doi: 10.1093/emboj/cdg222" |
