| Title: | Identification and Expression Profile of Chemosensory Receptor Genes in Aromia bungii (Faldermann) Antennal Transcriptome |
| Author(s): | Wu Z; Ye J; Qian J; Purba ER; Zhang Q; Zhang L; Mang D; |
| Address: | "Anhui Provincial Key Laboratory of Microbial Control, Engineering Research Center of Fungal Biotechnology, Ministry of Education, School of Forestry & Landscape Architecture, Anhui Agricultural University, Hefei 230036, China. Structural Cellular Biology Unit, Okinawa Institute of Science and Technology Graduate University, 1919-1 Tancha, Onna-Son 904-0495, Japan. Sterling International, Inc., Spokane, WA 99216, USA. Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Koganei 2-24-16, Tokyo 184-8588, Japan" |
| ISSN/ISBN: | 2075-4450 (Print) 2075-4450 (Electronic) 2075-4450 (Linking) |
| Abstract: | "The red-necked longicorn beetle, Aromia bungii (Faldermann) (Coleoptera: Cerambycidae), is a major destructive, wood-boring pest, which is widespread throughout the world. The sex pheromone of A. bungii was reported earlier; however, the chemosensory mechanism of the beetle remains almost unknown. In this study, 45 AbunORs, 6 AbunGRs and 2 AbunIRs were identified among 42,197 unigenes derived from the antennal transcriptome bioinformatic analysis of A. bungii adults. The sequence of putative Orco (AbunOR25) found in this study is highly conserved with the known Orcos from other Coleoptera species, and these Orco genes might be potentially used as target genes for the future development of novel and effective control strategies. Tissue expression analysis showed that 29 AbunOR genes were highly expressed in antennae, especially in the antennae of females, which was consistent with the idea that females might express more pheromone receptors for sensing pheromones, especially the sex pheromones produced by males. AbunOR5, 29, 31 and 37 were clustered with the pheromone receptors of the cerambycid Megacyllene caryae, suggesting that they might be putative pheromone receptors of A. bungii. All six AbunGRs were highly expressed in the mouthparts, indicating that these GRs may be involved in the taste perception process. Both AbunIRs were shown to be female-mouthparts-biased, suggesting that they might also be related to the tasting processes. Our study provides some basic information towards a deeper understanding of the chemosensing mechanism of A. bungii at a molecular level" |
| Keywords: | expression pattern gustatory receptor ionotropic receptor odorant receptor; |
| Notes: | "PubMed-not-MEDLINEWu, Zhenchen Ye, Jia Qian, Jiali Purba, Endang Rinawati Zhang, Qinghe Zhang, Longwa Mang, Dingze eng 31700569/National Natural Science Foundation of China/ Switzerland 2022/01/22 Insects. 2022 Jan 14; 13(1):96. doi: 10.3390/insects13010096" |
