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J Exp Biol

Title:		Identification and characterization of the bombykal receptor in the hawkmoth Manduca sexta
Author(s):		Wicher D; Morinaga S; Halty-deLeon L; Funk N; Hansson B; Touhara K; Stengl M;
Address:		"Max Planck Institute for Chemical Ecology, Department of Evolutionary Neuroethology, 07745 Jena, Germany dwicher@ice.mpg.de. Department of Applied Biological Chemistry, The University of Tokyo and JST ERATO Touhara Chemosensory Signal Project, Tokyo 113-8657, Japan. Max Planck Institute for Chemical Ecology, Department of Evolutionary Neuroethology, 07745 Jena, Germany. University of Kassel, Biology, Animal Physiology, 34132 Kassel, Germany"
Journal Title:		J Exp Biol
Year:		2017
Volume:		20170302
Issue:		Pt 10
Page Number:		1781 - 1786
DOI:		10.1242/jeb.154260
ISSN/ISBN:		1477-9145 (Electronic) 0022-0949 (Linking)
Abstract:		"Manduca sexta females attract their mates with the release of a species-specific sex-pheromone blend, with bombykal (E,Z)-10,12-hexadecadienal and (E,E,Z)-10,12,14-hexadecatrienal being the two major components. Here, we searched for the hawkmoth bombykal receptor in heterologous expression systems. The putative pheromone receptor MsexOr1 coexpressed with MsexOrco in Xenopus oocytes elicited dose-dependent inward currents upon bombykal application (10-300 mumol l(-1)), and coexpressed in HEK293 and CHO cells caused bombykal-dependent increases in the intracellular free Ca(2+) concentration. In addition, the bombykal receptor of Bombyx mori BmOr3 coexpressed with MsexOrco responded to bombykal (30-100 mumol l(-1)) with inward currents. In contrast, MsexOr4 coexpressed with MsexOrco responded neither to bombykal (30-100 mumol l(-1)) nor to the (E,E,Z)-10,12,14-hexadecatrienal mimic. Thus, MsexOr1, but not MsexOrco and probably not MsexOr4, is the bombykal-binding pheromone receptor in the hawkmoth. Finally, we obtained evidence that phospholipase C and protein kinase C activity are involved in the hawkmoth's bombykal-receptor-mediated Ca(2+) signals in HEK293 and CHO cells"
Keywords:		"Alkadienes/pharmacology Animals Bombyx Calcium Signaling Cricetulus HEK293 Cells Humans Manduca/cytology/*physiology Olfactory Receptor Neurons Oocytes *Receptors, Odorant Sex Attractants/*pharmacology Xenopus Manduca sexta Modulation Orco Pkc Plc Pheromo;"
Notes:		"MedlineWicher, Dieter Morinaga, Satoshi Halty-deLeon, Lorena Funk, Nico Hansson, Bill Touhara, Kazushige Stengl, Monika eng Research Support, Non-U.S. Gov't England 2017/03/04 J Exp Biol. 2017 May 15; 220(Pt 10):1781-1786. doi: 10.1242/jeb.154260. Epub 2017 Mar 2"