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Front Plant Sci

Title:		"MrTPS3 and MrTPS20 Are Responsible for beta-Caryophyllene and alpha-Pinene Production, Respectively, in Red Bayberry (Morella rubra)"
Author(s):		Wang Y; Yang Q; Zhu Y; Zhao L; Ju P; Wang G; Zhou C; Zhu C; Jia H; Jiao Y; Jia H; Gao Z;
Address:		"College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China. Key Laboratory for Silviculture and Conservation, Ministry of Education, Beijing Forestry University, Beijing, China. Yuyao Agriculture Technology Extension Center, Ningbo, China. Institute of Forestry, Ningbo Academy of Agricultural Science, Ningbo, China. College of Agronomy, Jiangxi Agricultural University, Nanchang, China"
Journal Title:		Front Plant Sci
Year:		2021
Volume:		20220107
Issue:
Page Number:		798086 -
DOI:		10.3389/fpls.2021.798086
ISSN/ISBN:		1664-462X (Print) 1664-462X (Electronic) 1664-462X (Linking)
Abstract:		"Red bayberry is a sweet, tart fruit native to China and grown widely in the south. The key organic compounds forming the distinctive aroma in red bayberry, are terpenoids, mainly beta-caryophyllene and alpha-pinene. However, the key genes responsible for different terpenoids are still unknown. Here, transcriptome analysis on samples from four cultivars, during fruit development, with different terpenoid production, provided candidate genes for volatile organic compound (VOC) production. Terpene synthases (TPS) are key enzymes regulating terpenoid biosynthesis, and 34 TPS family members were identified in the red bayberry genome. MrTPS3 in chromosome 2 and MrTPS20 in chromosome 7 were identified as key genes regulating beta-caryophyllene and alpha-pinene synthesis, respectively, by qRT-PCR. Subcellular localization and enzyme activity assay showed that MrTPS3 was responsible for beta-caryophyllene (sesquiterpenes) production and MrTPS20 for alpha-pinene (monoterpenes). Notably, one amino acid substitution between dark color cultivars and light color cultivars resulted in the loss of function of MrTPS3, causing the different beta-caryophyllene production. Our results lay the foundation to study volatile organic compounds (VOCs) in red bayberry and provide potential genes for molecular breeding"
Keywords:		Tps Voc functional differentiation transcriptome alpha-pinene beta-caryophyllene;
Notes:		"PubMed-not-MEDLINEWang, Yan Yang, Qinsong Zhu, Yifan Zhao, Lan Ju, Pengju Wang, Guoyun Zhou, Chaochao Zhu, Changqing Jia, Huijuan Jiao, Yun Jia, Huimin Gao, Zhongshan eng Switzerland 2022/01/25 Front Plant Sci. 2022 Jan 7; 12:798086. doi: 10.3389/fpls.2021.798086. eCollection 2021"