| Title: | Characterization of Two BAHD Acetyltransferases Highly Expressed in the Flowers of Jasminum sambac (L.) Aiton |
| Author(s): | Wang Y; Zhang H; Wan C; He X; Huang J; Lyu M; Yuan Y; Wu B; |
| Address: | "Ornamental Horticulture Department, College of Horticulture, Fujian Agriculture and Forestry University, No. 15, Shang Xia Dian Road, Cangshan District, Fuzhou 350002, China. The Fujian Provincial Key Laboratory of Plant Functional Biology, College of Life Sciences, Fujian Agriculture and Forestry University, No. 15, Shang Xia Dian Road, Cangshan District, Fuzhou 350002, China" |
| ISSN/ISBN: | 2223-7747 (Print) 2223-7747 (Electronic) 2223-7747 (Linking) |
| Abstract: | "Volatile benzenoid compounds are found in diverse aromatic bouquets emitted by most moth-pollinated flowers. The night-blooming Jasminum sambac is widely cultivated worldwide in the tropics and subtropics for ornamental and industrial purposes owing to its fragrant flowers. Benzylacetate is a characteristic constituent in jasmine scent which makes up to approximately 20-30% of the total emission in the headspace or extract, but the biosynthesis enzymes and the encoding genes have not yet been described. Here, we identify two cytosolic BAHD acyltransferases specifically expressed in the petals with a positive correlation closely to the emission pattern of the volatile benzenoids. Both JsBEAT1 and JsBEAT2 could use benzylalcohol and acetate-CoA as substrates to make benzylacetate in vitro. The recombinant GST-JsBEAT1 has an estimated apparent K(m) of 447.3 muM for benzylalcohol and 546.0 muM for acetate-CoA, whereas in the instance of the His-JsBEAT2, the K(m) values are marginally lower, being 278.7 and 317.3 muM, respectively. However, the catalytic reactions by the GST-JsBEAT1 are more efficient than that by the His-JsBEAT2, based on the steady-state k(cat) parameters. Furthermore, ectopic expression of JsBEAT1 and JsBEAT2 in the transgenic P. hybrida plants, driven by a flower-specific promotor, significantly enhances the biosynthesis of benzylbenzoate and benzylacetate, as well as the total VOCs" |
| Keywords: | BAHD acyltransferases family Jasminum sambac Oleaceae benzenoid benzyl acetate benzyl alcohol O-acetyltransferase (BEAT) enzyme kinetics floral scent; |
| Notes: | "PubMed-not-MEDLINEWang, Yuting Zhang, Hongliang Wan, Chao He, Xian Huang, Jinfeng Lyu, Meiling Yuan, Yuan Wu, Binghua eng 31902050/National Natural Science Foundation of China/ Switzerland 2022/01/12 Plants (Basel). 2021 Dec 21; 11(1):13. doi: 10.3390/plants11010013" |
