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Anal Chim Acta

Title:		High-pressure photon ionization time-of-flight mass spectrometry combined with dynamic purge-injection for rapid analysis of volatile metabolites in urine
Author(s):		Wang Y; Hua L; Jiang J; Xie Y; Hou K; Li Q; Wu C; Li H;
Address:		"Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, People's Republic of China; University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing 100039, People's Republic of China. Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, People's Republic of China. Department of Instrumentation and Electrical Engineering, Jilin University, Jilin 130021, People's Republic of China. Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, People's Republic of China. Electronic address: hli@dicp.ac.cn"
Journal Title:		Anal Chim Acta
Year:		2018
Volume:		1008
Issue:
Page Number:		74 - 81
DOI:		10.1016/j.aca.2018.01.006
ISSN/ISBN:		1873-4324 (Electronic) 0003-2670 (Linking)
Abstract:		"Small molecule metabolites are widely used as biomarkers in the research field of metabolomics for disease diagnosis and exposure assessment. As a readily available biofluid containing plenty of volatile organic metabolites (VOMs), urine is ideal for non-invasive metabolomic analysis; however, there is still lack of rapid analysis method for VOMs in urine. Here we report a kind of rapid method for urine analysis by employing high-pressure photon ionization time-of-flight mass spectrometry (HPPI-TOFMS) combined with dynamic purge-injection. Various types of metabolites, such as ketones, alcohols, acids, sulfides, pyrroles and amines were detected directly by simple acidification or alkalization of urines. It is noteworthy that nitrogen-containing compounds, especially polar amines, could be ultrasensitively measured without any derivatization. The analytical capability of the direct HPPI-MS technique was demonstrated by analyzing five valuable metabolites, i.e., toluene, 2,5-dimethylpyrrole, trimethlyamine, styrene, and p-xylene, which exhibited relatively low limits of detection, wide linear range and satisfactory repeatability. Being highly sensitive and humidity-friendly, the whole analytical procedure is easily operated in less than 6?ª+min. Interestingly, a new biomarker 2,5-dimethylpyrrole was exclusively found in the smoker's urine sample besides toluene. The work presents a novel tool for rapid nontarget disease biomarkers screening or target monitoring of specific compounds through the investigation of volatile metabolites in urine"
Keywords:		"Humans Mass Spectrometry *Photons Pressure Time Factors Volatile Organic Compounds/metabolism/*urine 2, 5-Dimethylpyrrole Dynamic purge-injection High-pressure photon ionization mass spectrometry Metabolomics Urine;"
Notes:		"MedlineWang, Yan Hua, Lei Jiang, Jichun Xie, Yuanyuan Hou, Keyong Li, Qingyun Wu, Chenxin Li, Haiyang eng Netherlands 2018/02/09 Anal Chim Acta. 2018 May 30; 1008:74-81. doi: 10.1016/j.aca.2018.01.006"