| Title: | Inositol is specifically involved in the sexual program of the fission yeast Schizosaccharomyces pombe |
| Author(s): | Voicu PM; Poitelea M; Schweingruber E; Rusu M; |
| Address: | "Department of Biochemistry, University of Medicine and Pharmacy Iasi, 6600 Iasi, Romania" |
| DOI: | 10.1007/s00203-001-0383-1 |
| ISSN/ISBN: | 0302-8933 (Print) 0302-8933 (Linking) |
| Abstract: | "The fission yeast Schizosaccharomyces pombe is a natural auxotroph for inositol and fails to grow in the complete absence of it. It was previously reported that a small concentration of inositol in the culture medium supports vegetative growth, but not mating and sporulation, and a tenfold of that concentration also supports mating and sporulation. The purpose of the present work was to investigate whether a moderate inositol starvation specifically affected events of the sexual program of development. A homothallic culture grown to the stationary phase in medium with a small inositol concentration was sterile but cells in the stationary phase of growth synchronously entered and completed the sexual cycle when inositol was added, without need of previous cell divisions. This suggests the involvement of inositol in a mechanism (or mechanisms) of the sexual program. The events of the program that were affected by inositol starvation were investigated. Commitment to mating and production of pheromone M were shown not to be inositol-dependent. A diploid strain homozygous at the mating-type locus and carrying a pat1-114 temperature-sensitive mutation in homozygous configuration sporulated under inositol starvation at the restrictive temperature; therefore starvation did not directly affect meiosis or sporulation. In contrast, production of pheromone P and the response of cells to pheromones were found to be inositol-dependent. The possibility that inositol or one of its derivative compounds is involved in pheromone P secretion and in pheromone signal reception is discussed" |
| Keywords: | "Culture Media Dose-Response Relationship, Drug Inositol/*pharmacology Pheromones/metabolism Reproduction Schizosaccharomyces/drug effects/growth & development/*physiology Spores, Fungal/drug effects/physiology;" |
| Notes: | "MedlineVoicu, Pia-Manuela Poitelea, Marius Schweingruber, Ernst Rusu, Mircea eng Research Support, Non-U.S. Gov't Germany 2002/03/22 Arch Microbiol. 2002 Mar; 177(3):251-8. doi: 10.1007/s00203-001-0383-1. Epub 2002 Jan 9" |
