| Title: | Fungistatic Activity Mediated by Volatile Organic Compounds Is Isolate-Dependent in Trichoderma sp. 'atroviride B' |
| Author(s): | van Zijll de Jong E; Kandula J; Rostas M; Kandula D; Hampton J; Mendoza-Mendoza A; |
| Address: | "Bio-Protection Research Centre, Lincoln University, Lincoln 7647, New Zealand. Linnaeus Laboratory Ltd., Gisborne 4010, New Zealand. Agricultural Entomology, Department of Crop Sciences, University of Gottingen, 37077 Gottingen, Germany. Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln 7647, New Zealand" |
| ISSN/ISBN: | 2309-608X (Electronic) 2309-608X (Linking) |
| Abstract: | "Trichoderma spp. produce multiple bioactive volatile organic compounds (VOCs). While the bioactivity of VOCs from different Trichoderma species is well documented, information on intraspecific variation is limited. The fungistatic activity of VOCs emitted by 59 Trichoderma sp. 'atroviride B' isolates against the pathogen Rhizoctonia solani was investigated. Eight isolates representing the two extremes of bioactivity against R. solani were also assessed against Alternaria radicina, Fusarium oxysporum f. sp. lycopersici and Sclerotinia sclerotiorum. VOCs profiles of these eight isolates were analyzed using gas chromatography-mass spectrometry (GC-MS) to identify a correlation between specific VOCs and bioactivity, and 11 VOCs were evaluated for bioactivity against the pathogens. Bioactivity against R. solani varied among the fifty-nine isolates, with five being strongly antagonistic. All eight selected isolates inhibited the growth of all four pathogens, with bioactivity being lowest against F. oxysporum f. sp. lycopersici. In total, 32 VOCs were detected, with individual isolates producing between 19 and 28 VOCs. There was a significant direct correlation between VOC number/quantity and bioactivity against R. solani. 6-pentyl-alpha-pyrone was the most abundant VOC produced, but 15 other VOCs were also correlated with bioactivity. All 11 VOCs tested inhibited R. solani growth, some by >50%. Some of the VOCs also inhibited the growth of the other pathogens by >50%. This study demonstrates significant intraspecific differences in VOC profiles and fungistatic activity supporting the existence of biological diversity within Trichoderma isolates from the same species, a factor in many cases ignored during the development of biological control agents" |
| Keywords: | Trichoderma sp.'atroviride B' biocontrol fungistatic activity volatile organic compounds; |
| Notes: | "PubMed-not-MEDLINEvan Zijll de Jong, Eline Kandula, Janaki Rostas, Michael Kandula, Diwakar Hampton, John Mendoza-Mendoza, Artemio eng LINX2201 ('Fungal organic volatile compounds for sustainable agriculture in a changing environment')/Ministry of Business, Innovation and Employment/ Smart Seeds/Ministry of Business, Innovation and Employment/ Bio-Protection Research Centre, Lincoln University, New Zealand./Tertiary Education Commission/ Switzerland 2023/02/26 J Fungi (Basel). 2023 Feb 10; 9(2):238. doi: 10.3390/jof9020238" |
