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Sensors (Basel)

Title:		Biogenic VOCs Emission Profiles Associated with Plant-Pest Interaction for Phenotyping Applications
Author(s):		Valencia-Ortiz M; Marzougui A; Zhang C; Bali S; Odubiyi S; Sathuvalli V; Bosque-Perez NA; Pumphrey MO; Sankaran S;
Address:		"Department of Biological System Engineering, Washington State University, Pullman, WA 99164, USA. Department of Plant Pathology, Washington State University, Pullman, WA 99164, USA. Department of Entomology, Plant Pathology and Nematology, University of Idaho, Moscow, ID 83844, USA. Department of Crop and Soil Science, Hermiston Agricultural Research & Extension Center, Oregon State University, Hermiston, OR 97838, USA. Department of Crop and Soil Sciences, Washington State University, Pullman, WA 99164, USA"
Journal Title:		Sensors (Basel)
Year:		2022
Volume:		20220628
Issue:		13
Page Number:		-
DOI:		10.3390/s22134870
ISSN/ISBN:		1424-8220 (Electronic) 1424-8220 (Linking)
Abstract:		"Pest attacks on plants can substantially change plants' volatile organic compounds (VOCs) emission profiles. Comparison of VOC emission profiles between non-infected/non-infested and infected/infested plants, as well as resistant and susceptible plant cultivars, may provide cues for a deeper understanding of plant-pest interactions and associated resistance. Furthermore, the identification of biomarkers-specific biogenic VOCs-associated with the resistance can serve as a non-destructive and rapid tool for phenotyping applications. This research aims to compare the VOCs emission profiles under diverse conditions to identify constitutive (also referred to as green VOCs) and induced (resulting from biotic/abiotic stress) VOCs released in potatoes and wheat. In the first study, wild potato Solanum bulbocastanum (accession# 22; SB22) was inoculated with Meloidogyne chitwoodi race 1 (Mc1), and Mc1 pathotype Roza (SB22 is resistant to Mc1 and susceptible to pathotype Roza), and VOCs emission profiles were collected using gas chromatography-flame ionization detection (GC-FID) at different time points. Similarly, in the second study, the VOCs emission profiles of resistant ('Hollis') and susceptible ('Alturas') wheat cultivars infested with Hessian fly insects were evaluated using the GC-FID system. In both studies, in addition to variable plant responses (susceptibility to pests), control treatments (non-inoculated or non-infested) were used to compare the VOCs emission profiles resulting from differences in stress conditions. The common VOC peaks (constitutive VOCs) between control and infected/infested samples, and unique VOC peaks (induced VOCs) presented only in infected/infested samples were analyzed. In the potato-nematode study, the highest unique peak was found two days after inoculation (DAI) for SB22 inoculated with Mc1 (resistance response). The most common VOC peaks in SB22 inoculated with both Mc1 and Roza were found at 5 and 10 DAI. In the wheat-insect study, only the Hollis showed unique VOC peaks. Interestingly, both cultivars released the same common VOCs between control and infected samples, with only a difference in VOC average peak intensity at 22.4 min retention time where the average intensity was 4.3 times higher in the infested samples of Hollis than infested samples of Alturas. These studies demonstrate the potential of plant VOCs to serve as a rapid phenotyping tool to assess resistance levels in different crops"
Keywords:		Animals Insecta Plants *Solanum tuberosum Triticum *Volatile Organic Compounds Gc-fid Hessian fly biomarkers biotic stress nematode potato wheat;
Notes:		"MedlineValencia-Ortiz, Milton Marzougui, Afef Zhang, Chongyuan Bali, Sapinder Odubiyi, Steven Sathuvalli, Vidyasagar Bosque-Perez, Nilsa A Pumphrey, Michael O Sankaran, Sindhuja eng 1011741/National Institute of Food and Agriculture/ 1014919/National Institute of Food and Agriculture/ College of Agricultural, Human, and Natural Resource Sciences Emerging Research Issues project/Washington State University/ Switzerland 2022/07/10 Sensors (Basel). 2022 Jun 28; 22(13):4870. doi: 10.3390/s22134870"