| Title: | "Determination of nicotine, tar, volatile organic compounds and carbonyls in mainstream cigarette smoke using a glass filter and a sorbent cartridge followed by the two-phase/one-pot elution method with carbon disulfide and methanol" |
| Author(s): | Uchiyama S; Hayashida H; Izu R; Inaba Y; Nakagome H; Kunugita N; |
| Address: | "Department of Environmental Health, National Institute of Public Health, 2-3-6, Minami, Wako City, Saitama 351-0197, Japan. Electronic address: uchiyama@niph.go.jp. Chiba University Graduate School of Engineering, 1-33, Yayoicho, Inage-ku, , Chiba-shi, Chiba 263-8522, Japan. Department of Environmental Health, National Institute of Public Health, 2-3-6, Minami, Wako City, Saitama 351-0197, Japan" |
| DOI: | 10.1016/j.chroma.2015.11.058 |
| ISSN/ISBN: | 1873-3778 (Electronic) 0021-9673 (Linking) |
| Abstract: | "We have developed a new analytical method for the determination of nicotine, tar, volatile organic compounds and carbonyls in main-stream cigarette smoke using a sorbent cartridge packed with Carboxen 572 (CX-572) and a Cambridge filter pad (CFP) followed by the two-phase/one-pot elution method. A CX-572 cartridge is installed between the intake of the CFP and the pump of the smoking machine. Gaseous compounds collected with the CX-572 cartridge and total particulate matter (TPM) collected with the CFP are coeluted simultaneously in the same vial and then analyzed by high-performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC/MS) and gas chromatograph-thermal conductivity detector (GC/TCD). Carbonyl compounds are determined by adding derivatizing reagent (2,4-dinitrophenylhydrazine, DNPH) to the eluate followed by HPLC analysis. VOCs and nicotine are determined by GC/MS, and water is determined by GC/TCD. The same sample eluate solution is used for HPLC, GC/MS and GC/TCD analyses. As a result of measuring main-stream cigarette smoke generated from reference cigarettes, almost all carbonyl compounds and VOCs except formaldehyde were passed through a CFP and trapped in a CX-572 cartridge. 100% of nicotine, tar and TPM were trapped in a CFP. 50% of water and 53% of formaldehyde were trapped in a CFP. The one-pot data is almost equal to the sums of CFP (particulate matter) and CX-572 (gaseous compounds) data. The two-phase/one-pot elution method can simultaneously measure nicotine, tar, volatile organic compounds and carbonyl compounds in cigarette smoke with simple operation and small amounts of reagents" |
| Keywords: | "Carbon Disulfide/chemistry Chemistry Techniques, Analytical/*instrumentation/*methods Chromatography, High Pressure Liquid Gas Chromatography-Mass Spectrometry Methanol/chemistry Nicotine/analysis Smoke/*analysis Tobacco/*chemistry Tobacco Products/analys;" |
| Notes: | "MedlineUchiyama, Shigehisa Hayashida, Hideki Izu, Rina Inaba, Yohei Nakagome, Hideki Kunugita, Naoki eng Research Support, Non-U.S. Gov't Netherlands 2015/12/15 J Chromatogr A. 2015 Dec 24; 1426:48-55. doi: 10.1016/j.chroma.2015.11.058. Epub 2015 Nov 19" |
