| Title: | Juvenile hormone regulation of HMG-R gene expression in the bark beetle Ips paraconfusus (Coleoptera: Scolytidae): implications for male aggregation pheromone biosynthesis |
| Author(s): | Tittiger C; Blomquist GJ; Ivarsson P; Borgeson CE; Seybold SJ; |
| Address: | "Department of Biochemistry/330, University of Nevada, Reno 89557, USA. crt@med.unr.edu" |
| ISSN/ISBN: | 1420-682X (Print) 1420-682X (Linking) |
| Abstract: | "Juvenile hormone III (JH III) induces acyclic isoprenoid pheromone production in male Ips paraconfusus. A likely regulatory enzyme in this process is 3-hydroxy-e-methylglutaryl-CoA reductase (HMG-R). To begin molecular studies on pheromone production, a 1.16-kb complementary DNA representing approximately one-third of I. paraconfusus HMG-R was isolated by polymerase chain reaction and sequenced. The predicted translation product is 59% and 75% identical to the corresponding portion of HMG-R from the fruit fly, Drosophila melanogaster, and the German cockroach, Blattella germanica, respectively. Northern blots show that topical application of JH III increases HMG-R transcript levels in male thoraces in an apparent dose- and time-dependent manner. These data support the model that JH III raises HMG-R transcript levels, resulting in increased activity of the isoprenoid pathway and de novo pheromone production" |
| Keywords: | "Amino Acid Sequence Animals Behavior, Animal/*physiology Cloning, Molecular Coleoptera/enzymology/*genetics Gene Expression Regulation, Enzymologic/*genetics Hydroxymethylglutaryl CoA Reductases/chemistry/*genetics Insect Proteins/chemistry/genetics Male;" |
| Notes: | "MedlineTittiger, C Blomquist, G J Ivarsson, P Borgeson, C E Seybold, S J eng Research Support, U.S. Gov't, Non-P.H.S. Switzerland 1999/03/05 Cell Mol Life Sci. 1999 Jan; 55(1):121-7. doi: 10.1007/s000180050275" |
