| Title: | Behavioral responses of Rhodnius prolixus to volatile organic compounds released in vitro by bacteria isolated from human facial skin |
| Author(s): | Tabares M; Ortiz M; Gonzalez M; Carazzone C; Vives Florez MJ; Molina J; |
| Address: | "Centro de Investigaciones Microbiologicas (CIMIC), Universidad de los Andes, Bogota, Colombia. Centro de Investigaciones en Microbiologia y Parasitologia Tropical (CIMPAT), Universidad de los Andes, Bogota, Colombia. Laboratory of Advanced Analytical Techniques in Natural Products (LATNAP), Universidad de los Andes, Bogota, Colombia" |
| DOI: | 10.1371/journal.pntd.0006423 |
| ISSN/ISBN: | 1935-2735 (Electronic) 1935-2727 (Print) 1935-2727 (Linking) |
| Abstract: | "BACKGROUND: Previous studies have demonstrated the role of volatile organic compounds (VOCs) produced by skin microbiota in the attraction of mosquitoes to humans. Recently, behavioral experiments confirmed the importance of VOCs released by skin microbiota in the attraction of Rhodnius prolixus (Hemiptera: Triatominae), a vector of Chagas disease. METHODS/FINDINGS: In this study, we screened for VOCs released in vitro by bacteria isolated from human facial skin that were able to elicit behavioral responses in R. prolixus. The VOCs released in vitro by eight bacterial species during two growth phases were tested with adult Rhodnius prolixus insects using a dual-choice 'T'-shaped olfactometer. In addition, the VOCs released by the bacteria were analyzed with headspace solid-phase microextraction gas chromatography-mass spectrometry (HS-SPME-GC-MS). The VOCs produced by Staphylococcus capitis 11C, Staphylococcus warneri and Staphylococcus epidermidis 1 were attractive to R. prolixus, while the VOCs released by Citrobacter koseri 6P, Brevibacterium epidermidis and Micrococcus luteus 23 were non-attractive. CONCLUSIONS: The results shown here indicate that VOCs released by bacteria isolated from human facial skin have a potential for biotechnological uses as a strategy to prevent the vectorial transmission of Chagas disease mediated by Rhodnius prolixus" |
| Keywords: | Animals Bacteria/*chemistry Chagas Disease/*parasitology Face/microbiology Female Gas Chromatography-Mass Spectrometry Humans Male *Microbiota Mosquito Vectors/*drug effects/physiology Rhodnius/*drug effects/physiology Skin/microbiology Trypanosoma cruzi/; |
| Notes: | "MedlineTabares, Marcela Ortiz, Mario Gonzalez, Mabel Carazzone, Chiara Vives Florez, Martha J Molina, Jorge eng Research Support, Non-U.S. Gov't 2018/04/24 PLoS Negl Trop Dis. 2018 Apr 23; 12(4):e0006423. doi: 10.1371/journal.pntd.0006423. eCollection 2018 Apr" |
