| Title: | Headspace-free setup of in vitro bioassays for the evaluation of volatile disinfection by-products |
| Author(s): | Stalter D; Dutt M; Escher BI; |
| Address: | "National Research Centre for Environmental Toxicology (Entox), The University of Queensland , 39 Kessels Road, Brisbane Qld 4108, Australia" |
| ISSN/ISBN: | 1520-5010 (Electronic) 0893-228X (Linking) |
| Abstract: | "The conventional setup of in vitro bioassays in microplates does not prevent the loss of volatile compounds, which hampers the toxicological characterization of waterborne volatile disinfection by-products (DBPs). To minimize the loss of volatile test chemicals, we adapted four in vitro bioassays to a headspace-free setup using eight volatile organic compounds (four trihalomethanes, 1,1-dichloroethene, bromoethane, and two haloacetonitriles) that cover a wide range of air-water partition coefficients. The nominal effect concentrations of the test chemicals decreased by up to three orders of magnitude when the conventional setup was changed to a headspace-free setup for the bacterial cytotoxicity assay using bioluminescence inhibition of Vibrio fischeri. The increase of apparent sensitivity correlated significantly with the air-water partition coefficient. Purge and trap GC/MS analysis revealed a reduced loss of dosed volatile compounds in the headspace free setup (78-130% of nominal concentration) compared to a substantial loss in the conventional set up (2-13% of the nominal concentration). The experimental effect concentrations converged with the headspace-free setup to the effect concentrations predicted by a QSAR model, confirming the suitability of the headspace-free approach to minimize the loss of volatile test chemicals. The analogue headspace-free design of the bacterial bioassays for genotoxicity (umuC assay) and mutagenicity (Ames fluctuation assay) increased the number of compounds detected as genotoxic or mutagenic from one to four and zero to two, respectively. In a bioassay with a mammalian cell line applied for detecting the induction of the Nrf-2-mediated oxidative stress response (AREc32 assay), the headspace-free setup improved the apparent sensitivity by less than one order of magnitude, presumably due to the retaining effect of the serum components in the medium, which is also reflected in the reduced aqueous concentrations of compounds. This study highlights the importance of adapting bioanalytical test setups when volatile/semivolatile compounds are present in the sample to avoid the loss of chemicals and thus to avoid underestimating the toxicity of mixtures and complex environmental samples" |
| Keywords: | "Air Aliivibrio fischeri/*drug effects/genetics/metabolism *Biological Assay Chemistry Techniques, Analytical/*methods Disinfectants/chemistry/metabolism/*toxicity Gas Chromatography-Mass Spectrometry Humans Luminescent Measurements MCF-7 Cells Mutagenicit;" |
| Notes: | "MedlineStalter, Daniel Dutt, Mriga Escher, Beate I eng Research Support, Non-U.S. Gov't 2013/10/15 Chem Res Toxicol. 2013 Nov 18; 26(11):1605-14. doi: 10.1021/tx400263h. Epub 2013 Oct 31" |
