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Mol Cell Biol

Title:		"Molecular analysis of SSN6, a gene functionally related to the SNF1 protein kinase of Saccharomyces cerevisiae"
Author(s):		Schultz J; Carlson M;
Address:		"Department of Genetics and Development, College of Physicians and Surgeons, Columbia University, New York, New York 10032"
Journal Title:		Mol Cell Biol
Year:		1987
Volume:		7
Issue:		10
Page Number:		3637 - 3645
DOI:		10.1128/mcb.7.10.3637-3645.1987
ISSN/ISBN:		0270-7306 (Print) 1098-5549 (Electronic) 0270-7306 (Linking)
Abstract:		"Mutations in the SSN6 gene suppress the invertase derepression defect caused by a lesion in the SNF1 protein kinase gene. We cloned the SSN6 gene of Saccharomyces cerevisiae and identified its 3.3-kilobase poly(A)-containing RNA. Disruption of the gene caused phenotypes similar to, but more severe than, those caused by missense mutations: high-level constitutivity for invertase, clumpiness, temperature-sensitive growth, alpha-specific mating defects, and failure to homozygous diploids to sporulate. In contrast, the presence of multiple copies of SSN6 interfered with derepression of invertase. An ssn6 mutation was also shown to cause glucose-insensitive expression of a GAL10-lacZ fusion and maltase. The mating defects of MAT alpha ssn6 strains were associated with production of two a-specific products, a-factor and barrier, and reduced levels of alpha-factor; no deficiency of MAT alpha 2 RNA was detected. We showed that ssn6 partially restored invertase expression in a cyr1-2 mutant, although ssn6 was clearly not epistatic to cyr1-2. We also determined the nucleotide sequence of SSN6, which is predicted to encode a 107-kilodalton protein with stretches of polyglutamine and poly(glutamine-alanine). Possible functions of the SSN6 product are discussed"
Keywords:		"Amino Acid Sequence Base Sequence Cloning, Molecular DNA Mutational Analysis Fungal Proteins/*genetics Gene Expression Regulation *Genes, Fungal Genetic Complementation Test Glucose/*physiology Glycoside Hydrolases/genetics Mating Factor Molecular Sequenc;"
Notes:		"MedlineSchultz, J Carlson, M eng GM34095/GM/NIGMS NIH HHS/ Research Support, U.S. Gov't, P.H.S. 1987/10/01 Mol Cell Biol. 1987 Oct; 7(10):3637-45. doi: 10.1128/mcb.7.10.3637-3645.1987"