| Title: | In vitro toxicological evaluation of surgical smoke from human tissue |
| Author(s): | Sisler JD; Shaffer J; Soo JC; LeBouf RF; Harper M; Qian Y; Lee T; |
| Address: | "1Pathology and Physiology Research Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, 1095 Willowdale Road, Morgantown, West Virginia 26505 USA. ISNI: 0000 0001 2163 0069. GRID: grid.416738.f 2Exposure Assessment Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, 1095 Willowdale Road, Morgantown, West Virginia 26505 USA. ISNI: 0000 0001 2163 0069. GRID: grid.416738.f 3Field Study Branch, Respiratory Health Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, 1095 Willowdale Road, Morgantown, West Virginia 26505 USA. ISNI: 0000 0001 2163 0069. GRID: grid.416738.f Zefon International, Inc., 5350 SW 1st Lane, Ocala, FL USA. 5Department of Environmental Engineering Sciences, University of Florida, Gainesville, FL USA. ISNI: 0000 0004 1936 8091. GRID: grid.15276.37" |
| DOI: | 10.1186/s12995-018-0193-x |
| ISSN/ISBN: | 1745-6673 (Print) 1745-6673 (Electronic) 1745-6673 (Linking) |
| Abstract: | "BACKGROUND: Operating room personnel have the potential to be exposed to surgical smoke, the by-product of using electrocautery or laser surgical device, on a daily basis. Surgical smoke is made up of both biological by-products and chemical pollutants that have been shown to cause eye, skin and pulmonary irritation. METHODS: In this study, surgical smoke was collected in real time in cell culture media by using an electrocautery surgical device to cut and coagulate human breast tissues. Airborne particle number concentration and particle distribution were determined by direct reading instruments. Airborne concentration of selected volatile organic compounds (VOCs) were determined by evacuated canisters. Head space analysis was conducted to quantify dissolved VOCs in cell culture medium. Human small airway epithelial cells (SAEC) and RAW 264.7 mouse macrophages (RAW) were exposed to surgical smoke in culture media for 24 h and then assayed for cell viability, lactate dehydrogenase (LDH) and superoxide production. RESULTS: Our results demonstrated that surgical smoke-generated from human breast tissues induced cytotoxicity and LDH increases in both the SAEC and RAW. However, surgical smoke did not induce superoxide production in the SAEC or RAW. CONCLUSION: These data suggest that the surgical smoke is cytotoxic in vitro and support the previously published data that the surgical smoke may be an occupational hazard to healthcare workers" |
| Keywords: | Healthcare workers Surgical smoke Toxicology; |
| Notes: | "PubMed-not-MEDLINESisler, Jennifer D Shaffer, Justine Soo, Jhy-Charm LeBouf, Ryan F Harper, Martin Qian, Yong Lee, Taekhee eng England 2018/04/06 J Occup Med Toxicol. 2018 Apr 2; 13:12. doi: 10.1186/s12995-018-0193-x. eCollection 2018" |
