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Plant Physiol Biochem

Title:		From plant resistance response to the discovery of antimicrobial compounds: The role of volatile organic compounds (VOCs) in grapevine downy mildew infection
Author(s):		Ricciardi V; Marciano D; Sargolzaei M; Maddalena G; Maghradze D; Tirelli A; Casati P; Bianco PA; Failla O; Fracassetti D; Toffolatti SL; De Lorenzis G;
Address:		"Department of Agricultural and Environmental Sciences, via Celoria 2, 20133, Milan, Italy. National Wine Agency of Georgia, Marshal Gelovani Avenue 6, 0159, Tbilisi, Georgia; Caucasus International University, Chargali str. 73, 0141, Tbilisi, Georgia. Department of Food Environmental and Nutritional Sciences, via Celoria 2, 20133, Milan, Italy. Department of Agricultural and Environmental Sciences, via Celoria 2, 20133, Milan, Italy. Electronic address: silvia.toffolatti@unimi.it. Department of Agricultural and Environmental Sciences, via Celoria 2, 20133, Milan, Italy. Electronic address: gabriella.delorenzis@unimi.it"
Journal Title:		Plant Physiol Biochem
Year:		2021
Volume:		20210126
Issue:
Page Number:		294 - 305
DOI:		10.1016/j.plaphy.2021.01.035
ISSN/ISBN:		1873-2690 (Electronic) 0981-9428 (Linking)
Abstract:		"The discovery of new mechanisms of resistance and natural bioactive molecules could be two of the possible ways to reduce fungicide use in vineyard and assure an acceptable and sustainable protection against Plasmopara viticola, the grapevine downy mildew agent. Emission of volatile organic compounds (VOCs), such as terpenes, norisoprenoids, alcohols and aldehydes, is frequently induced in plants in response to attack by pathogens, such as P. viticola, that is known to cause a VOCs increment in cultivars harboring American resistance traits. In this study, the role of leaf VOCs in the resistance mechanism of two resistant cultivars (Mgaloblishvili, a pure Vitis vinifera cultivar, and Bianca, an interspecific hybrid) and the direct antimicrobial activity of four selected VOCs have been investigated. The leaf VOCs profiles, analyzed through solid-phase microextraction gas chromatography-mass spectrometry analysis, as well as the expression of six terpene synthases (TPSs), were determined upon pathogen inoculation. In both cultivars, the expression pattern of six TPSs increased soon after pathogen inoculation and an increment of nine VOCs has been detected. While in Mgaloblishvili VOCs were synthesized early after P. viticola inoculation, they constituted a late response to pathogen in Bianca. All the four terpenes (farnesene, nerolidol, ocimene and valencene), chosen according to the VOC profiles and gene expression analysis, caused a significant reduction (53-100%) in P. viticola sporulation. These results support the role of VOCs into defense mechanisms of both cultivars and suggest their potential role as a natural and eco-friendly solution to protect grapevine from P. viticola"
Keywords:		"*Disease Resistance Fungicides, Industrial/chemistry Gene Expression Regulation, Plant Oomycetes/*pathogenicity Plant Diseases/*microbiology Vitis/*chemistry/microbiology Volatile Organic Compounds/*chemistry Disease containment Monoterpenes Plasmopara vi;"
Notes:		"MedlineRicciardi, Valentina Marciano, Demetrio Sargolzaei, Maryam Maddalena, Giuliana Maghradze, David Tirelli, Antonio Casati, Paola Bianco, Piero Attilio Failla, Osvaldo Fracassetti, Daniela Toffolatti, Silvia Laura De Lorenzis, Gabriella eng France 2021/02/05 Plant Physiol Biochem. 2021 Mar; 160:294-305. doi: 10.1016/j.plaphy.2021.01.035. Epub 2021 Jan 26"