| Title: | Characteristics of beta-glucosidase from oranges during maturation and its relationship with changes in bound volatile compounds |
| Author(s): | Ren JN; Yang ZY; Tai YN; Dong M; He MM; Fan G; |
| Address: | "Key Laboratory of Environment Correlative Dietology, Ministry of Education, College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China" |
| ISSN/ISBN: | 1097-0010 (Electronic) 0022-5142 (Linking) |
| Abstract: | "BACKGROUND: The hydrolysis of glycosidically bound volatile compounds can release potential aromas in oranges during maturation. beta-Glucosidase is the key enzyme that influences the hydrolysis of bound volatiles. In this study the changes in beta-glucosidase and bound volatile compounds in Jincheng oranges during maturation were investigated. The relationship between beta-glucosidase activity and bound volatiles was analyzed. RESULTS: The optimal temperature and pH of beta-glucosidase from Jincheng oranges were 40 degrees C and 5-6 respectively. Its Km and Vmax values were 0.61 mmol L(-1) and 0.009 U mg(-1) respectively. The activity of beta-glucosidase was strongly inhibited by Zn(2+), Fe(2+), Cu(2+), Ag(+), Hg(2+) and Fe(3+). beta-Glucosidase activity in pulp increased gradually during maturation, while that in peel first increased and then decreased in November. In total, 12 and 14 bound volatiles were found in pulp and peel respectively of this orange during maturation. CONCLUSION: The concentration of bound volatiles in pulp and peel decreased with the rise in beta-glucosidase activity in pulp and peel during maturation. This indicated that bound volatiles in Jincheng oranges were released during maturation owing to the increase in beta-glucosidase" |
| Keywords: | Citrus sinensis/*enzymology/growth & development/metabolism Fruit/*enzymology/growth & development/metabolism Gas Chromatography-Mass Spectrometry Humans Hydrogen-Ion Concentration Hydrolysis Kinetics Seasons Temperature Volatile Organic Compounds/*metabo; |
| Notes: | "MedlineRen, Jing-Nan Yang, Zi-Yu Tai, Ya-Nan Dong, Man He, Mang-Mang Fan, Gang eng Research Support, Non-U.S. Gov't England 2014/10/14 J Sci Food Agric. 2015 Aug 30; 95(11):2345-52. doi: 10.1002/jsfa.6956. Epub 2014 Nov 10" |
