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Nat Prod Res

Title:		Development and validation of headspace Solid-Phase microextraction coupled with gas chromatography (HS-SPME-GC) method for the analysis of zingiber zerumbet L
Author(s):		Bhavya ML; Ravi R; Madhava Naidu M;
Address:		"Spices, and Flavour science Department. Sensory Science Department, CSIR-Central Food Technological Research Institute, Mysore, Karnataka, 570 020, India"
Journal Title:		Nat Prod Res
Year:		2021
Volume:		20190722
Issue:		7
Page Number:		1221 - 1225
DOI:		10.1080/14786419.2019.1643863
ISSN/ISBN:		1478-6427 (Electronic) 1478-6419 (Linking)
Abstract:		"Headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography (GC-FID) was explored to determine the fingerprinting characteristic of Zingiber zerumbet L. volatiles to differentiate between different ginger species. The effect of different fibers [polydimethylsiloxane (PDMS, 7 mum), polyacrylate (PA, 85 mum)], different temperature and time on the HS-SPME-GC was investigated by using response surface methodology coupled with full factorial experimental design. The area percentage of the major sesquiterpenes (Zerumbone and alpha-humulene) were 56.3 +/- 4.7% and 47.5 +/- 27.2% with PA fiber, respectively at optimum condition of 70 degrees C and 30 min. Validation of the developed HS-SPME-GC method with limits of detection and quantification for zerumbone was 0.09 and 0.28 mug/g, respectively, demonstrating the suitable sensitivity of HS-SPME-GC method for the quantification of sesquiterpenes. Therefore, the simplicity of HS-SPME-GC makes it a convenient tool for qualitative and quantitative comparison of different ginger species targeting at the marker sesquiterpene molecule, zerumbone"
Keywords:		"Chromatography, Gas/*methods Dimethylpolysiloxanes/chemistry Gas Chromatography-Mass Spectrometry Reproducibility of Results Solid Phase Microextraction/*methods Volatile Organic Compounds/analysis Zingiberaceae/*chemistry Gas chromatography Headspace sol;"
Notes:		"MedlineBhavya, M L Ravi, R Madhava Naidu, M eng Validation Study England 2019/07/23 Nat Prod Res. 2021 Apr; 35(7):1221-1225. doi: 10.1080/14786419.2019.1643863. Epub 2019 Jul 22"