| Title: | [Impact of air quality on practices and results in the IVF laboratory] |
| Author(s): | Pollet-Villard X; Levy R; |
| Address: | "Nataliance, laboratoire Medibio, laboratoire BPR-as, LaboFrance, 755, avenue Jacqueline-Auriol, 45770 Saran, France. Electronic address: xavier.pollet.villard@gmail.com. Service de medecine de la reproduction-Cecos, hopital Tenon, HUEP, AP-HP, 4, rue de la Chine, 75020 Paris, France; UMRS 938, Sorbonne universites, universite Pierre-et-Marie-Curie (UPMC), universite Paris 06, 75012 Paris France" |
| Journal Title: | Gynecol Obstet Fertil Senol |
| DOI: | 10.1016/j.gofs.2018.09.011 |
| ISSN/ISBN: | 2468-7189 (Electronic) 2468-7189 (Linking) |
| Abstract: | "The concept of Air Quality often refers to particulate and microbiological contamination of ambiant air. European Directive 2006/86/CE encompass the IVF process and specify a class A air quality for manipulation of tissue and cells, in a class D environment (A over D rule). Recognizing the paramount importance of ensuring the highest microbiological and particulate safety in the IVF laboratory, it is equally important to take into account practicability issues and the financial burden of these recommendations, as well as the utter need to protect gametes and embryo viability during their IVF journey. The usefulness of such stringent recommendations may also be questionned given the absence of published cases of airborne contaminations and related patients infections after embryo transfer. The European directive stems from pharmaceutical standards and were not specifically designed for human IVF. Gametes and embryos are indeed extremely sensitive to physical and chemical stress and require strict temperature, osmolarity and pH control, as well as an absence of chemical contamination during manipulation and culture. These conditions are hardly obtained when using laminar flow hoods. Following concerns raised by many experts in the field, exceptions to the A over D rule were added in the 2006/86/CE Directives. This narrative review discusses all these aspects in a critical way and compare scientific and legal requirements applying to IVF practices in different regions of the world" |
| Keywords: | "Air Microbiology *Air Pollution Air Pollution, Indoor Embryo Transfer Embryo, Mammalian/physiology Female Fertilization in Vitro/*methods Humans Laboratories Male Oocytes/physiology Spermatozoa/physiology Amp Air quality Assisted reproductive technologies;" |
| Notes: | "MedlinePollet-Villard, X Levy, R fre Review France 2018/10/21 Gynecol Obstet Fertil Senol. 2018 Nov; 46(10-11):713-728. doi: 10.1016/j.gofs.2018.09.011. Epub 2018 Oct 16" |
