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J Dairy Res


Title:"Modification of the volatile compound profile of cheese, by a Lactococcus lactis strain expressing a mutant oligopeptide binding protein"
Author(s):Picon A; Fernandez-Garcia E; Gaya P; Nunez M;
Address:"Departamento de Tecnologia de Alimentos, SGIT-INIA, Carretera de La Coruna Km 7.5, 28040 Madrid Spain"
Journal Title:J Dairy Res
Year:2008
Volume:20080129
Issue:1
Page Number:30 - 36
DOI: 10.1017/S0022029907002981
ISSN/ISBN:0022-0299 (Print) 0022-0299 (Linking)
Abstract:"Lactococcus lactis strain AMP2I expresses OppA(D471R), a mutant oligopeptide binding OppA protein in which the aspartyl residue at position 471 was replaced by arginine. As a consequence of a different peptide transport in this strain, experimental Hispanico cheese made with Lc. lactis AMP2I had a higher content of total free amino acids than control cheese made with Lc. lactis AMP1I, an isogenic strain expressing wild-type OppA (Picon et al. 2005, 2007). In this work higher levels of diketones, hydroxy-ketones and, to a lesser extent, branched chain aldehydes were recorded for experimental cheese compared with control cheese. These differences levelled off as ripening proceeded. Strong correlations support the hypothesis that the increased levels of these volatile compounds in cheese made with Lc. lactis AMP2I are linked to higher concentrations of free amino acids threonine, valine and leucine"
Keywords:Bacterial Proteins/*genetics/*metabolism Carrier Proteins/*genetics/*metabolism Cheese/*analysis/microbiology Fermentation Food Microbiology Hydrogen-Ion Concentration Lactococcus lactis/genetics/*metabolism Lipoproteins/*genetics/*metabolism Mutation Oli;
Notes:"MedlinePicon, Antonia Fernandez-Garcia, Estrella Gaya, Pilar Nunez, Manuel eng England 2008/01/30 J Dairy Res. 2008 Feb; 75(1):30-6. doi: 10.1017/S0022029907002981. Epub 2008 Jan 29"

 
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