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Plant Physiol

Title:		NaJAZh regulates a subset of defense responses against herbivores and spontaneous leaf necrosis in Nicotiana attenuata plants
Author(s):		Oh Y; Baldwin IT; Galis I;
Address:		"Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Jena D-07745, Germany"
Journal Title:		Plant Physiol
Year:		2012
Volume:		20120409
Issue:		2
Page Number:		769 - 788
DOI:		10.1104/pp.112.193771
ISSN/ISBN:		1532-2548 (Electronic) 0032-0889 (Print) 0032-0889 (Linking)
Abstract:		"The JASMONATE ZIM DOMAIN (JAZ) proteins function as negative regulators of jasmonic acid signaling in plants. We cloned 12 JAZ genes from native tobacco (Nicotiana attenuata), including nine novel JAZs in tobacco, and examined their expression in plants that had leaves elicited by wounding or simulated herbivory. Most JAZ genes showed strong expression in the elicited leaves, but NaJAZg was mainly expressed in roots. Another novel herbivory-elicited gene, NaJAZh, was analyzed in detail. RNA interference suppression of this gene in inverted-repeat (ir)JAZh plants deregulated a specific branch of jasmonic acid-dependent direct and indirect defenses: irJAZh plants showed greater trypsin protease inhibitor activity, 17-hydroxygeranyllinalool diterpene glycosides accumulation, and emission of volatile organic compounds from leaves. Silencing of NaJAZh also revealed a novel cross talk in JAZ-regulated secondary metabolism, as irJAZh plants had significantly reduced nicotine levels. In addition, irJAZh spontaneously developed leaf necrosis during the transition to flowering. Because the lesions closely correlated with the elevated expression of programmed cell death genes and the accumulations of salicylic acid and hydrogen peroxide in the leaves, we propose a novel role of the NaJAZh protein as a repressor of necrosis and/or programmed cell death during plant development"
Keywords:		"Agrobacterium tumefaciens/genetics/metabolism Amino Acid Sequence Animals *Cell Death Cloning, Molecular Cyclopentanes/metabolism Gene Expression Regulation, Plant Genes, Plant *Herbivory Hydrogen Peroxide/metabolism Manduca/physiology Molecular Sequence;"
Notes:		"MedlineOh, Youngjoo Baldwin, Ian T Galis, Ivan eng 2012/04/13 Plant Physiol. 2012 Jun; 159(2):769-88. doi: 10.1104/pp.112.193771. Epub 2012 Apr 9"