| Title: | Comparison of headspace-oxylipin-volatilomes of some Eastern Himalayan mosses extracted by sample enrichment probe and analysed by gas chromatography-mass spectrometry |
| Author(s): | Mitra S; Burger BV; Poddar-Sarkar M; |
| Address: | "Post Graduate Department of Botany, Darjeeling Government College, Darjeeling, 734101, India. Department of Chemistry and Polymer Science, University of Stellenbosch, Private Bag X1, Matieland, 7602, South Africa. Department of Botany (Centre of Advanced Study), Chemical Signal and Lipidomics Laboratory, University of Calcutta, 35-Ballygunge Circular Road, Kolkata, 700019, India. mousumipsarkar1@gmail.com" |
| DOI: | 10.1007/s00709-016-1018-3 |
| ISSN/ISBN: | 1615-6102 (Electronic) 0033-183X (Linking) |
| Abstract: | "Mosses have an inherent adaptability against different biotic and abiotic stresses. Oxylipins, the volatile metabolites derived from polyunsaturated fatty acids (PUFAs), play a key role in the chemical defence strategy of mosses. In the present study, a comparative survey of these compounds, including an investigation into their precursor fatty acids (FAs), was carried out for the first time on the mosses Brachymenium capitulatum (Mitt.) Paris, Hydrogonium consanguineum (Thwaites & Mitt.) Hilp., Barbula hastata Mitt., and Octoblepharum albidum Hedw. collected from the Eastern Himalayan Biodiversity hotspot. Their headspace volatiles were sampled using a high-efficiency sample enrichment probe (SEP) and were characterized by gas chromatography-mass spectrometric analysis. FAs from neutral lipid (NL) and phospholipid (PL) fractions were also evaluated. Analysis of the oxylipin volatilome revealed the generation of diverse metabolites from C(5) to C(18), dominated by alkanes, alkenes, saturated and unsaturated alcohols, aldehydes, ketones and cyclic compounds, with pronounced structural variations. The C(6) and C(8) compounds dominated the total volatilome of all the samples. Analyses of FAs from membrane PL and storage NL highlighted the involvement of C(18) and C(20) PUFAs in oxylipin generation. The volatilome of each moss is characterized by a 'signature oxylipin mixture'. Quantitative differences in the C(6) and C(8) metabolites indicate their phylogenetic significance" |
| Keywords: | Bryophyta/*metabolism Fatty Acids/metabolism Gas Chromatography-Mass Spectrometry/*methods India *Metabolome Oxylipins/metabolism Phospholipids/metabolism Principal Component Analysis Volatile Organic Compounds/*metabolism Headspace Moss Oxylipin Polyunsa; |
| Notes: | "MedlineMitra, Souvik Burger, Barend V Poddar-Sarkar, Mousumi eng Comparative Study Austria 2016/10/06 Protoplasma. 2017 Mar; 254(2):1115-1126. doi: 10.1007/s00709-016-1018-3. Epub 2016 Oct 5" |
