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Mol Biol Rep

Title:		"Identification of odorant-binding protein genes from antennal expressed sequence tags of the onion fly, Delia antiqua"
Author(s):		Mitaka H; Matsuo T; Miura N; Ishikawa Y;
Address:		"Laboratory of Applied Entomology, Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo 113-8657, Japan"
Journal Title:		Mol Biol Rep
Year:		2011
Volume:		20100917
Issue:		3
Page Number:		1787 - 1792
DOI:		10.1007/s11033-010-0293-x
ISSN/ISBN:		1573-4978 (Electronic) 0301-4851 (Linking)
Abstract:		"Insect odorant-binding proteins (OBPs) are thought to play a crucial role in the chemosensation of hydrophobic molecules such as pheromones and host chemicals. The onion fly, Delia antiqua, is a specialist feeder of Allium plants, and utilizes a host odorant n-dipropyl disulfide as a cue for its oviposition. Because n-dipropyl disulfide is a highly hydrophobic compound, some OBPs might be indispensable for perception of it. However, no OBP gene has been identified in D. antiqua. Here, to obtain the DNA sequences of D. antiqua OBPs, we performed an analysis of antennal expressed sequence tags (ESTs). Among 288 EST clones, eight D. antiqua OBP genes were identified for the first time. Phylogenetic analysis revealed that each D. antiqua OBP gene is more closely related to its Drosophila orthologs than to the other D. antiqua OBP genes, suggesting that these OBP genes had emerged before the divergence of Delia and Drosophila species. All of the eight D. antiqua OBPs are expressed not only in the antennae but also in the legs, suggesting additional roles in the taste perception of non-volatile compounds. These findings serve as an important basis for understanding the molecular mechanisms underlying the host adaptations of D. antiqua"
Keywords:		"Animals Arthropod Antennae/*metabolism Diptera/*genetics Drosophila/genetics *Expressed Sequence Tags Gene Expression Regulation Genes, Insect/*genetics Insect Proteins/*genetics/metabolism Molecular Sequence Data Onions/*parasitology Phylogeny Receptors, ;"
Notes:		"MedlineMitaka, Hayato Matsuo, Takashi Miura, Nami Ishikawa, Yukio eng Netherlands 2010/09/18 Mol Biol Rep. 2011 Mar; 38(3):1787-92. doi: 10.1007/s11033-010-0293-x. Epub 2010 Sep 17"