| Title: | "A novel GTP-binding protein, Sar1p, is involved in transport from the endoplasmic reticulum to the Golgi apparatus" |
| Address: | "Department of Biology, Faculty of Science, University of Tokyo, Japan" |
| ISSN/ISBN: | 0021-9525 (Print) 1540-8140 (Electronic) 0021-9525 (Linking) |
| Abstract: | "SAR1, a gene that has been isolated as a multicopy suppressor of the yeast ER-Golgi transport mutant sec12, encodes a novel GTP-binding protein. Its nucleotide sequence predicts a 21-kD polypeptide that contains amino acid sequences highly homologous to GTP-binding domains of many ras-related proteins. Gene disruption experiments show that SAR1 is essential for cell growth. To test its function further, SAR1 has been placed under control of the GAL1 promoter and introduced into a haploid cell that had its chromosomal SAR1 copy disrupted. This mutant grows normally in galactose medium but arrests growth 12-15 h after transfer to glucose medium. At the same time, mutant cells accumulate ER precursor forms of a secretory pheromone, alpha-mating factor, and a vacuolar enzyme, carboxypeptidase Y. We propose that Sec12p and Sarlp collaborate in directing ER-Golgi protein transport" |
| Keywords: | "Amino Acid Sequence Base Sequence Blotting, Northern Blotting, Southern Cloning, Molecular Endoplasmic Reticulum/*metabolism Escherichia coli/*genetics/metabolism GTP-Binding Proteins/*genetics/metabolism Genetic Complementation Test Genotype Golgi Appara;" |
| Notes: | "MedlineNakano, A Muramatsu, M eng Comparative Study Research Support, Non-U.S. Gov't 1989/12/01 J Cell Biol. 1989 Dec; 109(6 Pt 1):2677-91. doi: 10.1083/jcb.109.6.2677" |
