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Photochem Photobiol Sci

Title:		AtBBX29 integrates photomorphogenesis and defense responses in Arabidopsis
Author(s):		Medina-Fraga AL; Chinen LA; Demkura PV; Lichy MZ; Gershenzon J; Ballare CL; Crocco CD;
Address:		"Facultad de Agronomia, IFEVA, Consejo Nacional de Investigaciones Cientificas y Tecnicas-Universidad de Buenos Aires, Av. San Martin 4453, C1417DSE, Ciudad Autonoma de Buenos Aires, Argentina. Department of Biochemistry, Max Planck Institute for Chemical Ecology, Jena, Germany. IIBIO, Consejo Nacional de Investigaciones Cientificas y Tecnicas-Universidad Nacional de San Martin, B1650HMP, Buenos Aires, Argentina. Facultad de Agronomia, IFEVA, Consejo Nacional de Investigaciones Cientificas y Tecnicas-Universidad de Buenos Aires, Av. San Martin 4453, C1417DSE, Ciudad Autonoma de Buenos Aires, Argentina. ccrocco@agro.uba.ar. Department of Plant Sciences, Section of Biology, Faculty of Sciences, University of Geneva, 1211, Geneva 4, Switzerland. ccrocco@agro.uba.ar"
Journal Title:		Photochem Photobiol Sci
Year:		2023
Volume:		20230218
Issue:		6
Page Number:		1475 - 1489
DOI:		10.1007/s43630-023-00391-8
ISSN/ISBN:		1474-9092 (Electronic) 1474-905X (Print) 1474-905X (Linking)
Abstract:		"Light is an environmental signal that modulates plant defenses against attackers. Recent research has focused on the effects of light on defense hormone signaling; however, the connections between light signaling pathways and the biosynthesis of specialized metabolites involved in plant defense have been relatively unexplored. Here, we show that Arabidopsis BBX29, a protein that belongs to the B-Box transcription factor (TF) family, integrates photomorphogenic signaling with defense responses by promoting flavonoid, sinapate and glucosinolate accumulation in Arabidopsis leaves. AtBBX29 transcript levels were up regulated by light, through photoreceptor signaling pathways. Genetic evidence indicated that AtBBX29 up-regulates MYB12 gene expression, a TF known to induce genes related to flavonoid biosynthesis in a light-dependent manner, and MYB34 and MYB51, which encode TFs involved in the regulation of glucosinolate biosynthesis. Thus, bbx29 knockout mutants displayed low expression levels of key genes of the flavonoid biosynthetic pathway, and the opposite was true in BBX29 overexpression lines. In agreement with the transcriptomic data, bbx29 mutant plants accumulated lower levels of kaempferol glucosides, sinapoyl malate, indol-3-ylmethyl glucosinolate (I3M), 4-methylsulfinylbutyl glucosinolate (4MSOB) and 3-methylthiopropyl glucosinolate (3MSP) in rosette leaves compared to the wild-type, and showed increased susceptibility to the necrotrophic fungus Botrytis cinerea and to the herbivore Spodoptera frugiperda. In contrast, BBX29 overexpressing plants displayed increased resistance to both attackers. In addition, we found that AtBBX29 plays an important role in mediating the effects of ultraviolet-B (UV-B) radiation on plant defense against B. cinerea. Taken together, these results suggest that AtBBX29 orchestrates the accumulation of specific light-induced metabolites and regulates Arabidopsis resistance against pathogens and herbivores"
Keywords:		*Arabidopsis/genetics/metabolism Glucosinolates *Arabidopsis Proteins/genetics/metabolism Mutation Flavonoids/pharmacology/metabolism Plant Diseases/microbiology Transcription Factors/genetics/metabolism/pharmacology Bbx Myb Plant defense response Plant p;
Notes:		"MedlineMedina-Fraga, Ana L Chinen, Lucas A Demkura, Patricia V Lichy, Micaela Z Gershenzon, Jonathan Ballare, Carlos L Crocco, Carlos D eng PICT-2018-4407/Agencia Nacional de Promocion de la Investigacion, el Desarollo Tecnologico y la Innovacion of Argentina/ PICT-2016-1711/Agencia Nacional de Promocion de la Investigacion, el Desarollo Tecnologico y la Innovacion of Argentina/ PICT-2018-02483/Agencia Nacional de Promocion de la Investigacion, el Desarrollo Tecnologico y la Innovacion/ PICT-2016-4490/Agencia Nacional de Promocion de la Investigacion, el Desarollo Tecnologico y la Innovacion of Argentina/ 31003A_132902/Swiss National Science Foundation fGrants/ England 2023/02/23 Photochem Photobiol Sci. 2023 Jun; 22(6):1475-1489. doi: 10.1007/s43630-023-00391-8. Epub 2023 Feb 18"