| Title: | Leaf herbivory counteracts nematode-triggered repression of jasmonate-related defenses in tomato roots |
| Author(s): | Martinez-Medina A; Mbaluto CM; Maedicke A; Weinhold A; Vergara F; van Dam NM; |
| Address: | "Molecular Interaction Ecology, German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig, Puschstrasse 4, 04103 Leipzig, Germany. Institute of Biodiversity, Friedrich Schiller University Jena, Dornburgerstrasse 159, 07743 Jena, Germany. Plant-Microorganism Interactions, Institute of Natural Resources and Agrobiology of Salamanca (IRNASA-CSIC), Cordel de Merinas 40-52, 37008 Salamanca, Spain" |
| ISSN/ISBN: | 1532-2548 (Electronic) 0032-0889 (Print) 0032-0889 (Linking) |
| Abstract: | "Shoot herbivores may influence the communities of herbivores associated with the roots via inducible defenses. However, the molecular mechanisms and hormonal signaling underpinning the systemic impact of leaf herbivory on root-induced responses against nematodes remain poorly understood. By using tomato (Solanum lycopersicum) as a model plant, we explored the impact of leaf herbivory by Manduca sexta on the performance of the root knot nematode Meloidogyne incognita. By performing glasshouse bioassays, we found that leaf herbivory reduced M. incognita performance in the roots. By analyzing the root expression profile of a set of oxylipin-related marker genes and jasmonate root content, we show that leaf herbivory systemically activates the 13-Lipoxigenase (LOX) and 9-LOX branches of the oxylipin pathway in roots and counteracts the M. incognita-triggered repression of the 13-LOX branch. By using untargeted metabolomics, we also found that leaf herbivory counteracts the M. incognita-mediated repression of putative root chemical defenses. To explore the signaling involved in this shoot-to-root interaction, we performed glasshouse bioassays with grafted plants compromised in jasmonate synthesis or perception, specifically in their shoots. We demonstrated the importance of an intact shoot jasmonate perception, whereas having an intact jasmonate biosynthesis pathway was not essential for this shoot-to-root interaction. Our results highlight the impact of leaf herbivory on the ability of M. incognita to manipulate root defenses and point to an important role for the jasmonate signaling pathway in shoot-to-root signaling" |
| Keywords: | Animals Cyclopentanes/*metabolism *Herbivory Solanum lycopersicum/*physiology Manduca/physiology Oxylipins/*metabolism Plant Growth Regulators/*metabolism Plant Leaves/*physiology Plant Roots/*physiology Tylenchoidea/physiology; |
| Notes: | "MedlineMartinez-Medina, Ainhoa Mbaluto, Crispus M Maedicke, Anne Weinhold, Alexander Vergara, Fredd van Dam, Nicole M eng Research Support, Non-U.S. Gov't 2021/10/08 Plant Physiol. 2021 Nov 3; 187(3):1762-1778. doi: 10.1093/plphys/kiab368" |
